HIGH EFFICIENCY MAPPING WITH A HUMAN MICROCELL PANEL

描述：（调查人员的摘要）在这项建议中， 描述了体细胞和分子遗传学方法， 产生用于映射的定义区域的起始材料， 人类基因组 一组完整的单染色体杂交体将在 通过将单个特定的人类染色体转移到 受体小鼠成纤维细胞。 该小组将提供给 它应该是克隆人类的重要工具， 人类基因序列以及人类的物理映射 基因组 条件也将确定为最有效的 利用微细胞杂种作为辐射杂种的供体细胞 映射. 辐射杂交将在各种细胞系中产生。 影响辐射杂种产生的所有参数的优化 也将受到考验。 此外，实验提出了开发一个 第二组单染色体杂交，专为 在辐射测绘实验中的应用。 有了这些资源， 不应该限制映射的可能性。

DESCRIPTION: (Investigators' Abstract) In this proposal a concerted somatic cell and molecular genetic approach is described which should result in starting materials for the mapping of defined regions of the human genome. A complete panel of monochromosomal hybrids will be generated by the transfer of single, specific human chromosomes into recipient mouse fibroblasts. This panel will be made available to interested investigators and should be an important tool for the cloning of human gene sequences as well as for the physical mapping of the human genome. Conditions will also be determined for the most efficient utilization of microcell hybrids as donor cells for radiation hybrid mapping. Radiation hybrids will be made in a variety of cell lines. Optimization of all parameters affecting generation of radiation hybrids will also be tested. Furthermore, experiments are proposed to develop a second panel of monochromosomal hybrids designed specifically for utilization in radiation mapping experiments. With these resources, there should be no limits to the possibilities of mapping.