LONG-TERM EFFECTS OF ANABOLIC STEROIDS IN MICE

合成代谢类固醇对小鼠的长期影响

• 批准号: 3331924
• 负责人: FRANKLIN H BRONSON
• 金额: $ 9.1万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1996-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3331924
• 关键词: aggression; aging; androgen analog; animal mortality; biological models; body composition; body movement; body physical activity; drug adverse effect; growth /development; laboratory mouse; longitudinal animal study; reproductive development; sex behavior

The use of androgenic-anabolic steroids is wide-spread among male athletes, and it is not uncommon among female athletes. While the immediate side effects of this practice are reasonably well documented, almost nothing is known about the long-term--i.e., lifetime--consequences of steroid abuse. This is a proposal to expose young adult male and female mice to androgenic-anabolic steroids and then survey the enduring consequences of this treatment in middle and old age. Specifically, starting at two months of age, CBF1 mice of both sexes will be given 6 months of cyclic exposure to either high or low doses of a "stacked" combination of four commonly-abused steroids. Some animals will be killed at 8, 12, or 24 months of age to look for effects on body composition, reproductive capability and the development of specific pathologies. Other mice will be tested at these same three ages to assess their spontaneous locomotor activity, strength and endurance, and sexual and aggressive behavior. These particular characteristics were chosen because they are all dependent to some degree on the organizing actions of androgen in normal males and the absence of these actions in normal females. Of particular importance in this set of experiments will be a careful documentation of any effect of steroid abuse on life span.

雄激素合成代谢类固醇的使用在男性中广泛传播 运动员，这在女性运动员中并不罕见。 而 这种做法的直接副作用是有据可查的， 几乎没有什么是关于长期的--即，终身-后果 类固醇滥用的证据 这是一个建议，暴露年轻的成年男性， 雌性小鼠雄激素合成类固醇，然后调查持久的 这种治疗在中年和老年的后果。 具体地说， 从两个月大开始，将给予两种性别的CBF 1小鼠6 几个月的周期性暴露于高剂量或低剂量的“堆叠” 四种常被滥用的类固醇 有些动物会 在8个月、12个月或24个月大时被杀死，以观察对身体的影响 组成、繁殖能力和特定的 病理学 其他小鼠将在这三个年龄进行测试， 评估其自发运动活动、力量和耐力，以及 性行为和攻击行为 这些特征是 之所以选择它们，是因为它们都在某种程度上依赖于组织 雄激素在正常男性中的作用，以及这些作用的缺乏， 正常的女性 在这一系列实验中， 仔细记录类固醇滥用对寿命的任何影响。