BULK SURFACE DISTRIBUTION OF CHOLESTERYL ESTERS

胆固醇酯的整体表面分布

• 批准号: 3337109
• 负责人: Howard L. Brockman
• 金额: $ 8.46万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3337109
• 关键词: acyl group; biomaterial interface interaction; calorimetry; chemical models; cholesterol; cholesterol esters; light scattering; lipid structure; mathematical model; membrane activity; molecular pathology; phase change; phospholipids; thermodynamics; triglycerides; unsaturated fatty acids; water

Long chain esters of cholesterol are very nonpolar lipids which, along with triglycerides, are mostly localized in bulk lipid phases like the cores of lipoproteins or arterial lipid deposits. These lipid droplets are separated from surrounding water by a monomolecular layer of amphipathic lipids which constitutes the site of cholesteryl ester mobilization. The long term goal of the project is to understand how the state and concentration of cholesteryl esters in interfaces is regulated by the composition and state of other lipid molecules in the bulk and surface phases. The immediate goals of this project are to understand how cholesteryl ester concentration and state is regulated in planar lipid films at the air/water interface and what regulates the partitioning of cholesteryl esters between these surfaces and bulk lipid phases. This is approached first by measurement and qualitative analysis of the composition dependence of the surface behavior of cholesteryl ester-containing mixtures. Based on these results mathematical models are derived and tested with the goal of understanding and predicting lipid phase distribution and state. Building on our existing knowledge we will determine on what basis different cholesteryl esters occupy available sites at the interface, i.e. how they compete with each other when partitioning from a bulk lipid phase. We will also study the competition between triglycerides and cholesteryl esters for the surface. Related to this will be investigations of the role of the physical state of the bulk phase and miscibility of components in regulating their availability at the surface. To model the effects of the adsorption of proteins and other water soluble constituents to the interface, we will study bile salt interactions with cholesteryl ester-containing films. These studies have direct relevance to cardiovascular pathogenesis and will provide a physical basis for the development of enzymatic techniques to remove arterial lipid deposits.

胆固醇的长链酯是非常非极性的脂质，其沿着 甘油三酸酯，主要定位在体脂质相中，如 脂蛋白或动脉脂质沉积。 这些脂滴是 通过单分子层的两亲性 脂质，其构成胆固醇酯移动的位点。 的 该项目的长期目标是了解国家和 界面中胆固醇酯的浓度由 其他脂质分子在本体和表面中的组成和状态 阶段。 本项目的直接目标是了解胆固醇酯 在空气/水的平面脂质膜中，浓度和状态被调节 界面和什么调节胆固醇酯之间的分配 这些表面和大量脂质相。 首先， 的组成依赖性的测量和定性分析， 含胆固醇酯混合物的表面行为。 基于这些 结果数学模型推导和测试的目标是 了解和预测脂相分布和状态。 根据我们现有的知识，我们将确定 不同的胆固醇酯占据界面处的可用位点，即， 当从大量脂质中分离时它们如何相互竞争 相位 我们还将研究甘油三酯之间的竞争， 胆固醇酯用于表面。 与此相关的将是调查 的作用的物理状态的体相和结晶性， 在调节其在表面的可用性的组件。 建模 蛋白质和其他水溶性成分的吸附作用 对于界面，我们将研究胆盐与胆固醇的相互作用 含酯薄膜。 这些研究与心血管发病机制直接相关， 为酶技术的发展提供了物理基础， 清除动脉脂质沉积。