NEURAL MECHANISMS OF CENTRAL CARDIOVASCULAR CONTROL

中枢心血管控制的神经机制

• 批准号: 3337516
• 负责人: JOHN B CABOT
• 金额: $ 12.94万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3337516
• 关键词: autoradiography; axon; baroreceptors; blood pressure; central nervous system; dorsal raphe nucleus; electron microscopy; electrophysiology; histochemistry /cytochemistry; neural conduction; neural information processing; neural inhibition; neuroanatomy; neurogenic hypertension; pigeons; radiotracer; reflex; sympathetic ganglion; sympathetic nervous system

Three series of investigations are proposed. The first involves a comprehensive anatomical and physiological examination of the serotonergic raphe-SPN pathway. Using autoradiography, horseradish peroxidase (HRP), lesion, microstimulation, and extracellular recording techniques, studies have been designed to: (a) localize the cells of origin and spinal trajectory of the raphe-SPN system; (b) establish the conduction velocities and degree of convergence and divergence of raphe axons terminating upon the SPNs; (c) investigate the spatial and temporal properties of the inhibition of SPN discharge associated with activation of this descending system; (d) assess the involvement of the raphe in baroreceptor and somatosympathetic reflexes; and (e) establish the role of the raphe in the "tonic" and "phasic" modulation of arterial blood pressure. The second involves anterograde transport studies to localize the cells of origin of the catecholaminergic input(s) upon the SPNs. Using autoradiography, the spinal projections of cells in the ventrolateral medullary region, locus coeruleus, and subcoeruleus would be described. Anterograde tract tracing would also be initiated on the paraventricular nucleus of the hypothalamus. The final project involves a preliminary electron microscopic description of the cytological characteristics of axon terminals on HRP-labelled preganglionic neurons.

提出了三个系列的调查。 第一个涉及全面的 肾上腺素能中缝-SPN通路的解剖学和生理学检查。 使用放射自显影、辣根过氧化物酶（HRP）、损伤、微刺激， 和细胞外记录技术，研究的目的是：（a） 定位中缝-SPN系统的起源细胞和脊柱轨迹;（B） 建立传导速度和收敛和发散的程度， 中缝轴突终止于SPNs;（c）调查空间和时间 与此激活相关的SPN放电的抑制特性 （d）评估中缝参与压力感受器， 躯体交感神经反射;和（e）建立中缝的作用， 动脉血压的“紧张性”和“阶段性”调节。 第二个涉及顺行运输研究，以定位细胞， SPN上的儿茶酚胺能输入的起源。 利用放射自显影术， 延髓腹外侧区细胞的脊髓投射， 蓝斑和蓝斑下核。 顺行道追踪将 也可以在下丘脑室旁核启动。 最后一个项目包括一个初步的电子显微镜描述的 HRP标记节前神经元轴突终末的细胞学特征 神经元