NEURAL MECHANISMS OF CENTRAL CARDIOVASCULAR CONTROL

提出了三个系列的调查建议。第一个涉及全面的 5-羟色胺能中缝-SPN通路的解剖学和生理学检查。 使用放射自显影，辣根过氧化物酶，损伤，微刺激， 和细胞外记录技术，研究的目的是：(A) 定位中缝SPN系统的起源细胞和脊髓轨迹；(B) 建立系统的传导速度、收敛和发散程度 中缝轴突终止于SPN；(C)研究空间和时间 激活SPN对SPN放电的抑制作用 下行系统；(D)评估中缝在压力感受器和 躯体交感神经反射；以及(E)确定中缝在 动脉血压的“紧张性”和“相位性”调节。 第二项研究涉及顺行运输研究，以定位脑膜瘤细胞 儿茶酚胺能传入孤束核的起源(S)使用放射自显影技术， 延髓腹外侧区细胞的脊髓投射 蓝斑和蓝斑亚核将被描述。顺行追踪将会 也可在下丘脑室旁核启动。 最后的项目包括初步的电子显微镜描述 HRP标记节前轴突终末的细胞学特征 神经元。

Three series of investigations are proposed. The first involves a comprehensive anatomical and physiological examination of the serotonergic raphe-SPN pathway. Using autoradiography, horseradish peroxidase (HRP), lesion, microstimulation, and extracellular recording techniques, studies have been designed to: (a) localize the cells of origin and spinal trajectory of the raphe-SPN system; (b) establish the conduction velocities and degree of convergence and divergence of raphe axons terminating upon the SPNs; (c) investigate the spatial and temporal properties of the inhibition of SPN discharge associated with activation of this descending system; (d) assess the involvement of the raphe in baroreceptor and somatosympathetic reflexes; and (e) establish the role of the raphe in the "tonic" and "phasic" modulation of arterial blood pressure. The second involves anterograde transport studies to localize the cells of origin of the catecholaminergic input(s) upon the SPNs. Using autoradiography, the spinal projections of cells in the ventrolateral medullary region, locus coeruleus, and subcoeruleus would be described. Anterograde tract tracing would also be initiated on the paraventricular nucleus of the hypothalamus. The final project involves a preliminary electron microscopic description of the cytological characteristics of axon terminals on HRP-labelled preganglionic neurons.