NUTRITIONAL EFFECTS ON NONHUMAN PRIMATE HDL

对非人类灵长类动物 HDL 的营养影响

• 批准号: 3337806
• 负责人: Lawrence L Rudel
• 金额: $ 26.16万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-08-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3337806
• 关键词: Cercopithecidae; affinity chromatography; alcoholic beverage consumption; animal colony; aorta; apolipoproteins; atherosclerosis; blood chemistry; blood lipoprotein metabolism; cholesterol; chylomicrons; coronary artery; density gradient ultracentrifugation; diet; dietary lipid; disease /disorder model; disease /disorder proneness /risk; enzyme linked immunosorbent assay; gel electrophoresis; high density lipoproteins; hyperlipoproteinemia; lipid metabolism; liver metabolism; low density lipoprotein; model design /development; nutrition related tag; pathogenic diet; phosphatidylcholines; protein metabolism; protein structure; radiotracer; saturated fat; triglycerides; unsaturated fats

Dietary polyunsaturated fat is believed to be beneficial for decreasing the risk of premature heart disease because it lowers plasma cholesterol concentrations. However, with this dietary fat high density lipoprotein (HDL) cholesterol concentration also decreases and low HDL cholesterol concentration is a risk factor for heart disease. We have shown that polyunsaturated fat fed monkeys had less atherosclerosis than saturated fat fed monkeys in spite of lower HDL concentration. The studies of this proposal are designed to determine if the total serum cholesterol lowering induced by a diet rich in monounsaturated fat is as beneficial in decreasing atherosclerosis in monkeys as that induced by a diet rich in polyunsaturated fat, since the former has been shown to lower low density lipoprotein (LDL) concentration without lowering that of HDL. In order to begin to understand the mechanisms of dietary fat modifications of plasma HDL concentration, the production of HDL particles by the isolated, perfused livers of monkeys will be studied. In monkeys, hepatic HDL particles are produced as precursor lipoproteins deficient in cholesterol esters. Using livers isolated from animals fed each type of dietary fat, we will measure the kinds of precursor particles produced as characterized by their apoprotein and lipid composition and their size. The amounts of the various precursor particles produced during liver perfusion will be measured as will the amounts of the individual HDL apoproteins. The conversion of each of the precursor lipoproteins into mature plasma HDL will be examined to determine their relationship to the plasma HDL subclasses. Detailed analyses of atherosclerosis will be made in each of the animals in which the plasma HDL and liver perfusate HDL have been analyzed. These studies will provide an opportunity to learn specific aspects of HDL metabolism important in atherosclerosis development in primates.

饮食中多不饱和脂肪被认为有益于减少 过早心脏病的风险降低了血浆胆固醇 浓度。 但是，使用这种饮食脂肪高密度脂蛋白 （HDL）胆固醇浓度也降低，HDL胆固醇低 浓度是心脏病的危险因素。 我们已经表明 多不饱和脂肪喂养的猴子的动脉粥样硬化少于饱和脂肪 尽管HDL浓度较低，喂养猴子。 这项研究 建议旨在确定总血清胆固醇是否降低 由富含单不饱和脂肪的饮食诱导的 减少猴子的动脉粥样硬化，如富含饮食所致 多不饱和脂肪，因为前者已显示出降低低密度 脂蛋白（LDL）浓度而不降低HDL的浓度。 为了 开始了解血浆饮食脂肪修饰的机制 HDL浓度，通过分离的HDL颗粒的产生， 将研究猴子的灌注肝脏。 在猴子中，肝HDL 颗粒作为前体脂蛋白缺乏胆固醇的前体脂蛋白 酯。 使用从饲喂每种饮食脂肪的动物中分离出的肝脏， 我们将测量以表征产生的前体颗粒的种类 通过其载脂蛋白和脂质成分及其大小。 金额 肝灌注过程中产生的各种前体颗粒将是 与单个HDL载蛋白的量一样测量。 这 将每种前体脂蛋白转化为成熟的血浆HDL 将检查以确定它们与血浆HDL的关系 子类。 将在每一个中进行详细的动脉粥样硬化分析 血浆HDL和肝灌注液HDL的动物已经 分析。 这些研究将为学习特定的机会提供机会 HDL代谢方面对动脉粥样硬化发展重要 灵长类动物。