RADIOASSAY OF ERYTHROPOIETIN

促红细胞生成素的放射测定

• 批准号: 3336879
• 负责人: GISELA K CLEMONS
• 金额: $ 16.28万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-08-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3336879
• 关键词: antibody formation; diagnosis design /evaluation; embryo /fetus; embryo /fetus hypoxia; erythropoiesis; erythropoietin; gestational diabetes mellitus; growth /development; hormone biosynthesis; hormone regulation /control mechanism; human pregnant subject; human subject; human tissue; hypoxia neonatorum; immunohematology; laboratory rabbit; laboratory rat; newborn animals; polycythemia; pregnancy toxemia /hypertension; radioimmunoassay; salivary glands

Erythropoietin is now accepted as the hormone controlling red blood cell production. A radioimmunoassay has been developed in this laboratory using pure radioiodine labeled erythropoietin and an anti-erythropoietin-antiserum prepared in rabbits. This assay measures not only normal circulating levels, but also depressed levels seen following physiological conditions known to depress erythropoiesis, and increased hormone levels associated with stimuli known to increase erythropoiesis, such as bleeding, hypoxia or cobalt administration. This radioimmunoassay shows a significant correlation with the in vivo bioassay for erythropoietin. Using this method, polycythemias of primary origin are distinguishable from those of secondary origin. The clinical significance of this proposal is derived from the diagnostic value of a sensitive assay of blood levels of erythropoietin. Clinical work proposed in this study deals with erythropoietin responses in infants born to diabetic or hypertensive mothers. Fetal hypoxemia in high risk pregnancies can be diagnosed by measuring the hormone level in the amniotic fluid. In addition to human erythropoietin a variety of animal erythropoietins are measurable with this assay. Animal work proposed is concerned with the ontogeny of Ep producing tissues in the fetal rat. The role of this hormone in neonatal erythropoiesis will be investigated as well as the site(s) of production and concentrations in response to anemia and hypoxia as a function of age and sex. The effects of exogenous Ep will be investigated in neonatal rabbits in order to gain information on whether the newborn animal metabolizes Ep differently than the adult. These studies may be of future clinical value in treatment of the anemia in premature infants with recombinant erythropoietin. Other animal studies are concerned with the effect of other hormones on renal erythropoietin synthesis and release, and with extrarenal sources of erythropoietin in adult animals. The possible function of high Ep levels found in rodent salivary glands will be further pursued. The knowledge gained from these investigations will add significantly to our understanding of the biogenesis and hormonal control of erythropoietin and to the role of this hormone in normal and diseased states in humans.

促红细胞生成素现在被认为是控制红细胞的激素 血细胞生成 放射免疫测定法已在 该实验室使用纯放射性碘标记的促红细胞生成素， 在兔中制备的抗促红细胞生成素抗血清。 该测定 不仅测量正常的循环水平， 在已知的生理条件下， 红细胞生成，以及与 已知增加红细胞生成的刺激物，如出血， 低氧或钴给药。 放射免疫分析显示， 与体内生物测定显著相关， 促红细胞生成素 使用这种方法，原发性红细胞增多症 起源与次级起源是有区别的。 的 这一建议的临床意义来自于 血液中的敏感检测的诊断价值 促红细胞生成素 本研究提出的临床工作涉及 促红细胞生成素反应在糖尿病或糖尿病 高血压母亲 高危妊娠的胎儿低氧血症 可以通过测量羊水中的激素水平来诊断 液 除了人促红细胞生成素外， 红细胞生成素可以用该测定法测量。 动物工作 提出的是关于产生EP的组织的个体发育 在胎鼠体内 这种激素在新生儿中的作用 将研究红细胞生成以及 响应贫血和缺氧的产生和浓度 as a function函数of age年龄and sex性别. 外源性Ep的作用将是 在新生兔中进行研究，以获得以下信息 新生动物是否代谢Ep不同于 成年人了 这些研究可能在未来的治疗中具有临床价值 的早产儿贫血与重组 促红细胞生成素 其他动物研究关注的是 其他激素对肾促红细胞生成素合成的影响， 成人促红细胞生成素的肾外来源 动物 啮齿类动物中发现的高Ep水平的可能功能 将进一步追踪唾液腺。 获得的知识 这些调查将大大增加我们 了解生物起源和激素控制 促红细胞生成素和这种激素在正常和 人类的病态