ENDOGENOUS OPIATE PEPTIDES AND CARDIOVASCULAR CONTROL

交感神经系统在脑血管疾病中的重要性 在有压力的情况下，心血管控制是不言而喻的。 最近，一种有效的交感神经活动调节系统 内源性阿片肽的研究已有报道。在 有意识的，完整的动物，这个系统显然与 在失血时限制交感神经的激活。虽然这件事 系统可以在各种压力情况下工作，我们的 调查将重点放在它在出血过程中的作用 低血压。一般的假设是内源性阿片类药物 中枢和外周多肽对自主神经的调节作用 神经系统功能，从而心血管控制在 交感神经活动增强的时期。我们将测试 以下具体假设：1)与低血压相关的 快速出血是由于激活了这种多肽能 系统以及由此引起的交感神经活性的降低； 2)心脏传入机制启动这种抑制 交感神经流出；(3)其他升压系统(如肾素- 血管紧张素和加压素)受此影响并与之相互作用 肽能降压系统；4)肾上腺释放 儿茶酚胺是由阿片肽通过 外围操作。由于内源性阿片肽的作用 会受到麻醉剂的影响，这些研究将在 留置动、静脉导管、血流探头和神经 记录电极。血浆和肾上腺儿茶酚胺将是 采用高效液相色谱-电化学检测法测定。建议数 研究是我们早期工作的自然延续。他们是 旨在了解这种肽能物质的作用 系统对交感神经活动的调节，因此， 心血管功能。

The importance of the sympathetic nervous system in cardiovascular control during stressful situations, is axiomatic. Recently, a potent system for modulation of sympathetic activity by endogenous opioid peptides has been described. In the conscious, intact animal, this system is apparently involved with limiting sympathetic activation during blood loss. Although this system may function in a variety of stressful situations, our investigation will focus on its role during hemorrhagic hypotension. The general hypothesis is that endogenous opioid peptides both centrally and peripherally modulate autonomic nervous system function and thus cardiovascular control during periods of elevated sympathetic activity. We will test the following specific hypotheses: 1) The hypotension associated with rapid hemorrhage results from activation of this peptidergic system and the resultant decrease in sympathetic nerve activity; 2) Cardiac afferent mechanisms initiate this depression of sympathetic outflow; (3) Other pressor systems (e.g., renin- angiotensin and vasopressin) are affected by and interact with this peptidergic depressor system; 4) Adrenal release of catecholamines is modulated by opioid peptides through a peripheral action. Since the effects of endogenous opioid peptides are affected by anesthetics, these studies will be performed with indwelling arterial and venous catheters, flow probes and nerve recording electrodes. Plasma and adrenal catecholamines will be measured by HPLC with electrochemical detection. The proposed studies are a natural continuation of our earlier work. They are directed toward an understanding of the role of this peptidergic system in modulation of sympathetic activity, and thus, cardiovascular function.