ENDOGENOUS OPIATE PEPTIDES AND CARDIOVASCULAR CONTROL

内源性阿片肽和心血管控制

• 批准号: 3342293
• 负责人: James C. Schadt
• 金额: $ 4.19万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3342293
• 关键词: adrenal medulla; autonomic ganglion; cardiovascular function; enkephalins; hemorrhage; high performance liquid chromatography; hypertension; laboratory rabbit; naloxone; neurotransmitters; norepinephrine; opioid receptor; radioimmunoassay; vascular resistance

The importance of the sympathetic nervous system in cardiovascular control during stressful situations, is axiomatic. Recently, a potent system for modulation of sympathetic activity by endogenous opioid peptides has been described. In the conscious, intact animal, this system is apparently involved with limiting sympathetic activation during blood loss. Although this system may function in a variety of stressful situations, our investigation will focus on its role during hemorrhagic hypotension. The general hypothesis is that endogenous opioid peptides both centrally and peripherally modulate autonomic nervous system function and thus cardiovascular control during periods of elevated sympathetic activity. We will test the following specific hypotheses: 1) The hypotension associated with rapid hemorrhage results from activation of this peptidergic system and the resultant decrease in sympathetic nerve activity; 2) Cardiac afferent mechanisms initiate this depression of sympathetic outflow; (3) Other pressor systems (e.g., renin- angiotensin and vasopressin) are affected by and interact with this peptidergic depressor system; 4) Adrenal release of catecholamines is modulated by opioid peptides through a peripheral action. Since the effects of endogenous opioid peptides are affected by anesthetics, these studies will be performed with indwelling arterial and venous catheters, flow probes and nerve recording electrodes. Plasma and adrenal catecholamines will be measured by HPLC with electrochemical detection. The proposed studies are a natural continuation of our earlier work. They are directed toward an understanding of the role of this peptidergic system in modulation of sympathetic activity, and thus, cardiovascular function.

交感神经系统的重要性 心血管控制在紧张的情况下，是不言自明的。 最近，一种有效的交感神经活动调节系统， 已经描述了内源性阿片肽。 在 有意识的，完整的动物，这个系统显然与 限制了失血期间的交感神经激活。 虽然这 系统可以在各种压力情况下发挥作用，我们 研究将集中在它在出血过程中的作用 低血压 一般的假设是内源性阿片样物质 中枢和外周肽调节自主神经 神经系统功能和心血管控制 交感神经活动增强的时期 我们将测试 以下具体假设：1）与低血压相关的 这种肽能物质的激活会导致快速出血 系统和交感神经活动的减少; 2)心脏传入机制启动这种抑制， 交感神经流出;（3）其他升压系统（例如，肾素， 血管紧张素和血管加压素）受此影响并与其相互作用 肽能降压系统; 4）肾上腺释放 类阿片肽通过一种 外围动作 由于内源性阿片肽的作用 受麻醉剂影响，这些研究将在 留置动脉和静脉导管、流量探头和神经 记录电极 血浆和肾上腺儿茶酚胺将 通过具有电化学检测的HPLC测量。 拟议 研究是我们早期工作的自然延续。 他们是 旨在了解这种肽能的作用， 系统调节交感神经活动，因此， 心血管功能