DIETARY SALT AND BLOOD PRESSURE REGULATION

膳食盐和血压调节

• 批准号: 3340996
• 负责人: Silvia H Azar
• 金额: $ 9.29万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1985-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3340996
• 关键词: blood pressure; cardiovascular disorder prevention; dietary excess; dietary mineral; embryo /fetus; environment associated hypertension; familial hypertension; homeostasis; mother /embryo /fetus nutrition; nutrition related tag; saluresis

These studies will investigate the effects of maternal dietary salt excess, on BP development in offspring of genetically hypertensive rats, postulating that: the development and severity of hypertension in a susceptible individual could be modified by salt excess acting during pre-natal and early post-natal life. A hypertension susceptible mother with an excessive salt intake transmits to the offspring via milk, a factor(s) which in interaction with renal immaturity aggrevates offspring hypertension. After weaning, different environments associated with the offspring's new stage of life modulate the final course of BP through maturity. To test this hypothesis we will use a cross-suckling design and multivariate analysis. The spontaneously hypertensive rat (SHR) and the control Wistar-Kyoto rat (WKY) will be used; the environmental factor will be NaC1 in the diet of mother, nurse and weanling. The diet salt content (low 0.3% and high 4%) will be manipulated at three spans: pregnancy, lactation, and post-weanling stage. SHR and WKY dams will be pair-matched for BP and randomly assigned to 0.3% or 4% salt diets for one week (4 groups) prior to mating and maintained on their assigned diets until delivery. Then one "environmental" and one "genetic" manipulation will be done. Each mother will be a nurse and her diet will be randomly assigned to either low or high salt (8 groups). Half the male pups of each strain will then be cross-suckled (16 groups). All pups will be weaned at 19 days, and the pup's diet randomized to high or low salt (32 groups). Maternal endpoints will be: body weight, BP, heart rate, food and water intake (days 7, 15, and 20 post-mating, and 7 and 18 post-partum); circadian milk Na, K, water, fat, protein, and lactose content (days 7 and 19 post-partum). Offsprings end point will be: milk "intake" at 7 days by tritiaded water administration to the mother; body weight, BP, heart rate, plasma volume, carcass Na, K, water content and renal studies (kidney weight, DNA/protein ratio, microscopy and glomerular number) at 0 and 7 days; plasma volume, BP and renal studies at 19 days and BP and renal studies on 140 days. Postweanling pups will have BP measurements every 2-4 weeks for one year. Micropuncture will be used to measure BP, heart rate and plasma volume in sucklings; established methods will be used for glomerular count, kidney DNA/protein ratio, and renal morphology. This proposal will dissect the role of maternal environment and genetics in the development of body fluids, kidney and hypertension, which will provide a rationale for preventative measures in the hypertension prone individual.

这些研究将调查母亲饮食中盐分过量的影响， 遗传性高血压大鼠子代血压发育的研究， 假设：老年人高血压的发展和严重程度 敏感个体可能会因盐过量作用而改变 出生前和出生后早期的生活。易患高血压的母亲 摄入过多的盐分会通过牛奶传递给后代， 肾不成熟与子代聚集相互作用的S因子 高血压。断奶后，与之相关的不同环境 后代的新生命阶段通过调整BP的最终进程 成熟。为了验证这一假设，我们将使用交叉哺乳设计 多变量分析。自发性高血压大鼠(SHR)和 将使用控制Wistar-京都鼠(WKY)；环境因素将 在母亲、护士和断奶婴儿的饮食中加入NaCl.饮食中的盐分含量 (低0.3%和高4%)将在三个跨度内进行操作：怀孕， 哺乳期和断奶后阶段。SHR和WKY大坝将配对 对于BP，随机分配到0.3%或4%的食盐饮食一周(4 群体)在交配之前，并维持在他们分配的食物中，直到 送货。那么一个“环境”操作和一个“基因”操作将是 搞定了。每个母亲都是一名护士，她的饮食将被随机分配 至低盐或高盐(8组)。每一品系的雄性幼崽的一半 然后将被交叉哺乳(16组)。所有幼崽将在19岁断奶 将幼犬的饮食随机分为高盐或低盐两组(32组)。 产妇的终点是：体重、血压、心率、食物和水。 采食量(交配后7、15和20天，产后7和18天)； 昼夜乳钠、钾、水分、脂肪、蛋白质和乳糖含量(第7天和 产后19例)。后代的终点将是：在7天前摄取乳汁 给母亲服用三联水；体重，血压，心率， 血浆容量、屠体钠、钾、水分含量和肾脏检查(肾脏 体重、DNA/蛋白质比率、显微镜和肾小球数量)在0和7 天数；19天时的血浆量、血压和肾脏检查以及血压和肾脏 为期140天的研究。断奶后的幼崽将每2-4次测量一次血压 一年中的几周。微穿刺术将被用来测量血压、心率 和乳汁中的血浆体积；已建立的方法将用于 肾小球计数、肾脏DNA/蛋白质比值和肾脏形态。这 该提案将剖析母体环境和遗传学在母体发育中的作用 发展体液，肾脏和高血压，这将提供一个 有高血压倾向的个体采取预防措施的理由。