CARBON DIOXIDE TRANSPORT AND CHEMOSENSITIVITY

二氧化碳传输和化学敏感性

• 批准号: 3340503
• 负责人: JEROME M ADAMS
• 金额: $ 3.74万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3340503
• 关键词: acid base balance; anions; brain stem; carbon dioxide transport; carbonate dehydratase; carbonic anhydrase inhibitors; chemoreceptors; computer data analysis; homeostasis; ion transport; membrane permeability; pulmonary respiration

An increase in the carbon dioxide concentration in arterial blood caused by disease or altered environment has a profound effect on breathing. The brain stem chemoreceptors thought to be primarily responsible are stimulated by hydrogen ion changes resulting from the reaction of CO2 with water in the tissue. This study is based on the hypothesis that catalysis of CO2 hydration by carbonic anhydrase in glial cells and transport of the resulting bicarbonate ions across the cellmembranes by a specific anion carrier system are cardinal steps in the function of the chemoreceptors. We propose to evaluate this hypothesis by experiments in anesthetized rats to measure the respiratory response to CO2. Inhibitors of catalysis and bicarbonate transport will be administered by perfusing the cerebrospinal fluid space of the brain stem. The parameters of a theoretical model of CO2 transport which includes the hydration reaction and carrier mediated HCO3 transport can then be statistically estimated from the experimental data and compared before and after inhibition of catalysis and transport. The results of this study will provide an understanding of the early events in the homeostasis of the brain acid-base environment and its effects on control of breathing.

动脉血中二氧化碳浓度的增加， 疾病或改变的环境对呼吸有深远的影响。 的 脑干化学感受器被认为是主要负责 受CO2与CO2反应产生的氢离子变化的刺激， 组织中的水。 这项研究是基于这样一个假设， 神经胶质细胞中碳酸酐酶对CO2水合作用的影响以及 产生的碳酸氢根离子通过特定的阴离子穿过细胞膜 载体系统是化学感受器功能的重要步骤。 我们建议通过麻醉大鼠的实验来评估这一假设 来测量对二氧化碳的呼吸反应。 催化抑制剂和 碳酸氢盐转运将通过灌注脑脊髓 脑干的液体空间 理论模型的参数 CO2传输包括水合反应和载体介导 HCO3运输可以从实验数据中进行统计估计。 数据，并比较催化和转运抑制前后。 这项研究的结果将提供对早期事件的了解 在大脑酸碱环境的内稳态中的作用及其对 控制呼吸。