PLASMA FIBRONECTIN IN PLATELET HEMOSTATIC FUNCTION
PLASMA FIBRONECTIN IN PLATELET HEMOSTATIC FUNCTION
批准号:
3339659
负责人:
Mark HOWARD Ginsberg
金额:
$13.17万
依托单位国家:
美国
项目类别:
财政年份:
1982
资助国家:
美国
项目状态:
已结题
起止时间:
1982-01-01 至 1986-12-31
关键词:
autoradiography cell adhesion cell cell interaction crosslink cytoskeleton disulfide bond electrofocusing fibrinogen gel electrophoresis hemostasis hemostatics human tissue hybridomas immunofluorescence technique membrane activity monoclonal antibody monocyte platelet aggregation radiotracer spectrometry thrombin
中文摘要
血小板在止血和血栓栓塞性疾病中起核心作用
英文摘要
Platelets play a central role in hemostasis and thromboembolic disease, in
part adherence to each other (aggregation) or vessel walls (adhesion).
These reactions are mediated in part by interactions with a class of large
disulfide-linked glycoproteins. We have found that thrombin induces
specific and saturable binding of one of these glycoproteins, fibronectin
(fn) to platelets. Based on our preliminary data, we envision the
interaction of fn with the platelet to involve the four steps diagrammed
below. Resting Cell greater than FN Binding Site II greater than Fn
Binding III greater than Fn Processing IV greater than Functional. We will
dissect and define each of these individual steps in the reaction
sequence. Reaction I describes the induction of the fn binding site by
platelet stimulation. We shall identify stimuli which do and do not
support fn binding using both secretory and non secretory stimuli. The
effect of selected drugs on fn binding will be investigated as a means of
defining biochemical pathways required for binding site induction.
Reaction II entails fn binding to the platelet. The effect of
environmental conditions such as temperature, pH and divalent ions, will be
established, and the domains of fn which are recognized by the platelet
will be defined. In addition, multiple approaches will be used to identify
the binding site for fn. Particular emphasis will be given to the role of
fibrin(ogen) in fn binding based on our preliminary data. Reaction III
entails processing of platelet-bound fn, and we shall investigate
cross-linking of fn on the platelet surface via transglutaminases or
disulfide bonding, internalization of fn, and interactions with
cytoskeletal elements. Reaction IV involves the functional consequences of
fn binding to the platelet, either as a result of initial binding or
subsequent processing. Its effect on platelet aggregation and adhesion,
clot retraction and monocyte-platelet interaction represent likely
candidate events which will be explored.
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会议论文
Cellular Mechanisms of Inflammation, Hemostasis, and Thrombosis
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批准号:10229365
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项目类别:
-
资助金额:$234.03万
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财政年份:2020
-
负责人:Mark HOWARD Ginsberg
-
依托单位:
Direct Rap1-talin interaction in platelets, leukocytes, and endothelial cells
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批准号:10229368
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项目类别:
-
资助金额:$48.79万
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财政年份:2020
-
负责人:Mark HOWARD Ginsberg
-
依托单位:
Cellular Mechanisms of Inflammation, Hemostasis, and Thrombosis
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批准号:10676869
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项目类别:
-
资助金额:$233.35万
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财政年份:2020
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负责人:Mark HOWARD Ginsberg
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依托单位:
Core B - Ginsberg-ADMINISTRATIVE CORE
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批准号:10676887
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项目类别:
-
资助金额:$7.86万
-
财政年份:2020
-
负责人:Mark HOWARD Ginsberg
-
依托单位:
Core B - Ginsberg-ADMINISTRATIVE CORE
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批准号:10229366
-
项目类别:
-
资助金额:$7.95万
-
财政年份:2020
-
负责人:Mark HOWARD Ginsberg
-
依托单位:
Direct Rap1-talin interaction in platelets, leukocytes, and endothelial cells
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批准号:10676892
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项目类别:
-
资助金额:$48.69万
-
财政年份:2020
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负责人:Mark HOWARD Ginsberg
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依托单位:
Deconvoluting the Vascular Adhesome
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批准号:10327637
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项目类别:
-
资助金额:$78.7万
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财政年份:2018
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负责人:Mark HOWARD Ginsberg
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依托单位:
Deconvoluting the Vascular Adhesome
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批准号:10548841
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项目类别:
-
资助金额:$78.7万
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财政年份:2018
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负责人:Mark HOWARD Ginsberg
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依托单位:
Anti-Coagulant and Cytoprotective activity in CCM pathogenesis
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批准号:10417155
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项目类别:
-
资助金额:$31.45万
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财政年份:2015
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负责人:Mark HOWARD Ginsberg
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依托单位:
Anti-Coagulant and Cytoprotective activity in CCM pathogenesis
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批准号:10621253
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项目类别:
-
资助金额:$31.38万
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财政年份:2015
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负责人:Mark HOWARD Ginsberg
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依托单位:
Anti-Coagulant and Cytoprotective activity in CCM pathogenesis
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批准号:10220146
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项目类别:
-
资助金额:$31.52万
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财政年份:2015
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负责人:Mark HOWARD Ginsberg
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依托单位:
Composition and Functions of the Integrin Activation Complex
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批准号:8695078
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项目类别:
-
资助金额:$38.75万
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财政年份:2014
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负责人:Mark HOWARD Ginsberg
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依托单位:
Project 4: A Binary Switch in Adhesion Maturation
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批准号:8234230
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项目类别:
-
资助金额:$26.99万
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财政年份:2011
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负责人:Mark HOWARD Ginsberg
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依托单位:
Administrative Core
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批准号:8256553
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项目类别:
-
资助金额:$9.43万
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财政年份:2011
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负责人:Mark HOWARD Ginsberg
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依托单位:
KRIT1 and Vascular Integrity
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批准号:8038085
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项目类别:
-
资助金额:$38.63万
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财政年份:2011
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负责人:Mark HOWARD Ginsberg
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依托单位:
Activation of B3 Integrins
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批准号:8256548
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项目类别:
-
资助金额:$47.35万
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财政年份:2011
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负责人:Mark HOWARD Ginsberg
-
依托单位:
KRIT1 and Vascular Integrity
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批准号:8207881
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项目类别:
-
资助金额:$38.69万
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财政年份:2011
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负责人:Mark HOWARD Ginsberg
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依托单位:
KRIT1 and Vascular Integrity
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批准号:8605067
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项目类别:
-
资助金额:$37.98万
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财政年份:2011
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负责人:Mark HOWARD Ginsberg
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依托单位:
KRIT1 and Vascular Integrity
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批准号:8402853
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项目类别:
-
资助金额:$36.89万
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财政年份:2011
-
负责人:Mark HOWARD Ginsberg
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依托单位:
Activation of B3 Integrins
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批准号:7995808
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项目类别:
-
资助金额:$47.89万
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财政年份:2010
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负责人:Mark HOWARD Ginsberg
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依托单位:
国内基金
海外基金
GMFG/F-actin/cell adhesion 轴驱动 EHT 在造
血干细胞生成中的作用及机制研究
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批准号:TGY24H080011
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项目类别:省市级项目
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资助金额:--
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批准年份:2024
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负责人:李鸿鹄
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依托单位: