ROLE OF C3A/C5A IN ANTIGEN-INDUCED BRONCHOCONSTRICTION
ROLE OF C3A/C5A IN ANTIGEN-INDUCED BRONCHOCONSTRICTION
批准号:
3339812
负责人:
JEAN F. REGAL
金额:
$8.0万
依托单位国家:
美国
项目类别:
财政年份:
1981
资助国家:
美国
项目状态:
已结题
起止时间:
1981-07-01 至 1987-06-30
中文摘要
在以前的研究中,我们已经证明了C5a,一个裂解产物的第五
英文摘要
In previous studies, we have shown that C5a, a cleavage product of the fifth
complement, causes a significant contraction of the isolated guinea pig trachea
which is mediated by a product (SRS-A) or products of arachidonate metabolism.
The proposed research is intended to extend these previous studies to test the
hypothesis that C3a, a cleavage product of the third component of complement,
and/or C5a cause tracheal contraction and bronchoconstriction in the guinea pig
via the same mediator or mediators as does antigen in sensitized guinea pigs.
C3a and C5a will be purified from guinea pig serum by established
chromatographic procedures. Mediators of antigen induced changes in airway
caliber will be investigated in animals passively sensitized with IgG type
anti-ovalbumin as well as animals passively sensitized with only IgE type
anti-ovalbumin. IgG and IgE will be separated using protein A-Sepharose CL-4B
affinity chromatography and will be assayed using passive cutaneous anaphylaxis.
Two models for examining changes in airway caliber will be utilized:
contraction of isolated guinea pig tracheal smooth muscle and in vivo
measurements of lung resistance and dynamic compliance on inhalation challenge
in the guinea pig. Pharmacological agents will be utilized to investigate the
role of histamine and products of arachiodonate metabolism in C5a, C3a and
antigen induced tracheal contraction and bronchoconstriction. In addition, such
antagonists will be used to investigate the role of prostaglandins as modulators
of histamine involvement in the responses. Crosstachyphylaxis between antigen
or C3a/C5a induced tracheal contraction and bronchoconstriction will also be
examined. If the results of the proposed studies indicate that C3a and/or C5a
are possible mediators of antigen induced bronchoconstriction, by virtue of
similar antagonism by pharmacological agents, more long term studies will then
be conducted to look for the formation of C3a and/or C5a in antigen induced
bronchoconstriction.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Complement activation and angiogenic imbalance in pregnancy and hypertension
-
批准号:8179940
-
项目类别:
-
资助金额:$44.57万
-
财政年份:2011
-
负责人:JEAN F. REGAL
-
依托单位:
Complement Activation in Pregnancy and Hypertension
-
批准号:8574333
-
项目类别:
-
资助金额:$43.72万
-
财政年份:2011
-
负责人:JEAN F. REGAL
-
依托单位:
IMMUNOTOXICITY OF ACID ANHYDRIDES IN THE LUNG
-
批准号:2459007
-
项目类别:
-
资助金额:$18.05万
-
财政年份:1996
-
负责人:JEAN F. REGAL
-
依托单位:
IMMUNOTOXICITY OF ACID ANHYDRIDES IN THE LUNG
-
批准号:2156803
-
项目类别:
-
资助金额:$19.5万
-
财政年份:1996
-
负责人:JEAN F. REGAL
-
依托单位:
IMMUNOTOXICITY OF ACID ANHYDRIDES IN THE LUNG
-
批准号:2749675
-
项目类别:
-
资助金额:$18.77万
-
财政年份:1996
-
负责人:JEAN F. REGAL
-
依托单位:
IMMUNOTOXICITY OF ACID ANHYDRIDES IN THE LUNG
-
批准号:6043474
-
项目类别:
-
资助金额:$19.52万
-
财政年份:1996
-
负责人:JEAN F. REGAL
-
依托单位:
ROLE OF C3A/C5A IN ANTIGEN-INDUCED BRONCHOCONSTRICTION
-
批准号:3339806
-
项目类别:
-
资助金额:$12.76万
-
财政年份:1981
-
负责人:JEAN F. REGAL
-
依托单位:
ROLE OF C3A/C5A IN ANTIGEN-INDUCED BRONCHOCONSTRICTION
-
批准号:3339813
-
项目类别:
-
资助金额:$9.91万
-
财政年份:1981
-
负责人:JEAN F. REGAL
-
依托单位:
ROLE OF C3A/C5A IN ANTIGEN-INDUCED BRONCHOCONSTRICTION
-
批准号:3339814
-
项目类别:
-
资助金额:$10.14万
-
财政年份:1981
-
负责人:JEAN F. REGAL
-
依托单位:
ROLE OF C3A/C5A IN ANTIGEN-INDUCED BRONCHOCONSTRICTION
-
批准号:3339811
-
项目类别:
-
资助金额:$8.13万
-
财政年份:1981
-
负责人:JEAN F. REGAL
-
依托单位:
海外基金