SUBENDOTHELIAL BINDING OF VON WILLEBRAND FACTOR

血管内皮损伤导致血小板快速黏附 裸露的内皮下层，这是 止血和动脉粥样硬化形成的过程。这种血小板黏附需要 Von Willebrand因子是一种多聚体血浆蛋白，它的存在 我们已经证明也存在于血管内皮下。我们 计划确定内皮下结合血管的消化研究，通过 检测VWF与纯化的已知内皮下取代基的结合 并通过免疫电子显微镜定位技术。我们也希望 以确定绑定中是否存在多聚体优先级 血管内皮细胞因子的亚组分。阐明VWF的结合作用 对内皮下层的研究将是理解 血小板黏附的机制。

Injury to vascular endothelium results in the rapid adhesion of platelets to the exposed underlying subendothelium, an important step in the processes of hemostasis and atherogenesis. This platelet adhesion requires the presence of von Willebrand factor, a multimeric plasma protein, which we have demonstrated also to be present within vascular subendothelium. We plan to identify the subendothelial binding vessel digestion studies, by examining the binding of VWF to purified known subendothelial substituents and by immuno-electron microscopic localization techniques. We also wish to determine whether there is a multimeric preferentiality in the binding of VWF to subendothelial constitutents. Elucidation of the binding of VWF to subendothelium would be an important advance in understanding the mechanism of platelet adhesion.