NEUROTRANSMITTERS AND CENTRAL CONTROL OF BLOOD PRESSURE

描述(调查人员摘要)：监管中的骚乱 血压可能是由于体内递质机制的扰动所致。 初级终末的孤束核(NTS) 心血管传入神经。因此，对推定人所发挥的作用的定义 NTS中的发射器可能会增加对中央控制的理解 血压。首席调查员此前曾表示， 甘氨酸(Gly)在NTS中释放。微量注射氨基酸 进入背侧NTS会引起降压和心动过缓反应 定性上与兴奋性氨基酸谷氨酸(GLU)相似，而 迷走神经腹侧NTS和背侧运动核(DMV)的注射 导致升压，心动过速反应。来自调查员的研究 实验室支持ACH的角色，但不支持 甘氨酸-谷氨酸受体在上述过程中的相互作用 心血管反应。调查员将检验这一假设 甘氨酸通过胆碱能引起NTS的心血管反应 机制，特别是通过释放ACh。甘氨酸在人类免疫系统中的作用 心血管反射中的NTS将被测试，并从 将确定新界南区在功能上不同的地点。的作用 将评估ACh在调节Gly反应中的作用及其机制 GLY导致ACh释放的方式将被确定。免疫细胞化学 将使用方法来识别和本地化含有Gly的终端和 Gly受体及其与胆碱能关系的研究 将对神经元和神经突起进行研究。最后， GLY终末与心血管传入纤维的关系 被调查以确定是否可以从初级传入神经释放GLY 或来自NTS内的中间神经元。

DESCRIPTION (Investigator's Abstract): Disturbances in the regulation of blood pressure may result from perturbations of transmitter mechanisms in the nucleus tractus solitarius (NTS), the site of termination of primary cardiovascular afferents. Thus, definition of the role played by putative transmitters in the NTS may increase understanding of central control of blood pressure. The principal investigator had previously shown that glycine (GLY) is released in the NTS. Micorinjection of the amino acid into the dorsal NTS elicits a depressor and bradycardiac response that is qualitatively like that of the excitatory amino acid glutamate (GLU), while injections into the ventral NTS and dorsal motor nucleus of the vagus (DMV) result in pressor, tachycardiac responses. Studies from the investigator's laboratory have supported a role for ACh, and have not supported glycine-glutamate receptor interactions in the mediation of these cardiovascular responses. The investigator will test the hypothesis that GLY elicits cardiovascular responses from the NTS through cholinergic mechanisms and specifically through the release of ACh. The role of GLY in the NTS in cardiovascular reflexes will be tested and projections from the functionally different sites in the NTS will be determined. The role of ACh in mediating the responses to GLY will be assessed and the mechanisms by which GLY leads to ACh release will be determined. Immunocytochemical methods will be used to identify and localize GLY-containing terminals and GLY receptors, and the relationship of those GLY elements to cholinergic neurons and nerve processes will be investigated. Finally, the relationship between GLY terminals and cardiovascular afferent fibers will be investigated to determine if GLY may be released from primary afferents or from interneurons within the NTS.