SUBSTANCE P AND HYPERTENSION

本课题将研究神经肽P物质在中枢神经系统中的作用 高血压的调节和这一系统可能的异常 高血压。该项目包括4个领域，具体如下 目标： 第一区：在正常和自发性高血压大鼠身上进行研究 第四脑室和核内给药的影响 与P物质相关的多肽、酶的孤立束 处理P物质和针对这些酶的抗血清。 区域II：分离、纯化和鉴定加工过程中的酶 P物质在大鼠脑内的分布。 第三区：制备针对该病毒的单抗和多克隆抗体 在大鼠大脑中处理P物质的酶。 第四区：进行免疫细胞化学研究以定位该物质 中枢神经系统中P加工酶的光镜和电子显微镜水平 正常大鼠和高血压大鼠。开发测量酶的方法。 P物质似乎是一种重要的神经递质，调节 血压，包括传入和传出水平。P物质出现 仅在中枢神经系统发挥神经递质和/或神经调质作用 经过产生N-末端或C-末端的酶加工后 碎片。这些酶将被表征、定位和量化。 在正常和自发性高血压大鼠中。

This project will study the role of the neuropeptide substance P in CNS regulation of blood pressure and possible abnormalities of this system in hypertension. This project comprises 4 Areas, with the following specific aims: Area I: Conduct studies in normal and spontaneously hypertensive rats on effects of administration into the fourth ventricle and into the nucleus tractus solitarius of peptides related to Substance P, of enzymes which process Substance P and of antisera directed against these enzymes. Area II: Isolate, purify and characterize the enzymes which process Substance P in rat brain. Area III: Raise monoclonal and polyclonal antibodies directed against the enzymes which process Substance P in rat brain. Area IV: Conduct immunocytochemical studies to localize the Substance P-processing enzymes at the light and electron microscopic level in the CNS of normal and hypertensive rats. To develop methods to measure the enzymes. Substance P appears to be an essential neurotransmitter for regulation of blood pressure, both at afferent and efferent levels. Substance P appears to exert its neurotransmitter and/or neuromodulator effects in the CNS only after processing by enzymes which produce N-terminal or C-terminal fragments. These enzymes will be characterized, localized and quantitated in normal and spontaneously hypertensive rats.