SUBSTANCE P AND HYPERTENSION

P 物质与高血压

• 批准号: 3345499
• 负责人: JOHN M STEWART
• 金额: $ 23.68万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-09-30 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3345499
• 关键词: affinity chromatography; antibody formation; baroreceptors; blood pressure; central nervous system; electron microscopy; ethylenediaminetetraacetate; gel filtration chromatography; high performance liquid chromatography; histochemistry /cytochemistry; hypertension; immunochemistry; immunofluorescence technique; ion exchange chromatography; microinjections; monoclonal antibody; neurogenic hypertension; stereotaxic techniques

This project will study the role of the neuropeptide substance P in CNS regulation of blood pressure and possible abnormalities of this system in hypertension. This project comprises 4 Areas, with the following specific aims: Area I: Conduct studies in normal and spontaneously hypertensive rats on effects of administration into the fourth ventricle and into the nucleus tractus solitarius of peptides related to Substance P, of enzymes which process Substance P and of antisera directed against these enzymes. Area II: Isolate, purify and characterize the enzymes which process Substance P in rat brain. Area III: Raise monoclonal and polyclonal antibodies directed against the enzymes which process Substance P in rat brain. Area IV: Conduct immunocytochemical studies to localize the Substance P-processing enzymes at the light and electron microscopic level in the CNS of normal and hypertensive rats. To develop methods to measure the enzymes. Substance P appears to be an essential neurotransmitter for regulation of blood pressure, both at afferent and efferent levels. Substance P appears to exert its neurotransmitter and/or neuromodulator effects in the CNS only after processing by enzymes which produce N-terminal or C-terminal fragments. These enzymes will be characterized, localized and quantitated in normal and spontaneously hypertensive rats.

本课题研究神经肽P物质在中枢神经系统中的作用 调节血压和这个系统可能的异常， 高血压 该项目包括4个领域，具体如下 目的： 领域I：在正常和自发性高血压大鼠中进行以下研究： 第四脑室和核内给药的影响 与P物质有关的肽的孤束， 处理P物质和针对这些酶的抗血清。 区域II：分离、纯化和表征加工的酶 大鼠脑内P物质含量。 区域III：产生针对 老鼠大脑中处理P物质的酶。 区域IV：进行免疫细胞化学研究以定位物质 中枢神经系统中光学和电子显微镜水平的P-加工酶 正常和高血压大鼠的血压。 开发测量酶的方法。 P物质似乎是一种重要的神经递质， 血压，在传入和传出水平。 P物质似乎 仅在CNS中发挥其神经递质和/或神经调质作用 在通过产生N-末端或C-末端的酶处理后， 片段 这些酶将被表征、定位和定量 在正常和自发性高血压大鼠中。