IMMUNOLOGICAL STUDIES OF ANGIOTENSIN CONVERTING ENZYME

本课题旨在研究免疫损伤血管的机制， 并开发肺内皮损伤的测定。 人血管紧张素转换酶，其是血管内皮细胞的一种成分， 内皮细胞膜，将被纯化。 纯化的酶将被 用于开发酶联免疫测定法，以检测循环 进行性系统性红斑狼疮患者的抗体 系统性硬化症和成人呼吸窘迫综合征。 多克隆和 将产生血管紧张素转化酶的单克隆抗体。 这些将被测试它们与膜结合酶的相互作用， 人内皮细胞培养。 血小板活化因子释放和 将测量凝血活性。 将检测单克隆抗体 因为它们对肺血管紧张素转换酶具有特异性。 一 将使用特异性单克隆抗体测定循环 内皮损伤时肺酶释放。

This proposal is to study mechanisms of immunological injury to vascular endothelium and to develop an assay for pulmonary endothelial injury. Human angiotensin converting enzyme, which is a constituent of the luminal membrane of endothelium, will be purified. The purified enzyme will be used to develop an enzyme-linked immunoassay to detect circulating antibodies in patients with systemic lupus erythematosus, progressive systemic sclerosis and adult respiratory distress syndrome. Polyclonal and monoclonal antibodies to angiotensin converting enzyme will be produced. These will be tested for their interaction with membrane bound enzyme in human endothelial cell culture. Platelet activating factor release and coagulant activity will be measured. Monoclonal antibodies will be tested for their specificity for pulmonary angiotensin converting enzyme. A specific monoclonal antibody will be used to assay for circulating pulmonary enzyme released during endothelial injury.