IMMUNOLOGICAL STUDIES OF ANGIOTENSIN CONVERTING ENZYME

本研究拟对免疫损伤血管的机制进行研究。 并建立一种肺内皮损伤的检测方法。 人血管紧张素转换酶，它是鲁米那的一种成分 内皮细胞膜，将被提纯。纯化的酶将是 用于开发检测循环的酶联免疫分析 进行性系统性红斑狼疮患者的抗体 系统性硬化症和成人呼吸窘迫综合征。多克隆和 将产生抗血管紧张素转换酶的单抗。 这些将测试它们与膜结合酶的相互作用 人内皮细胞培养。血小板活化因子释放和 将测量凝血剂的活性。将对单抗进行检测 因其对肺血管紧张素转换酶的特异性。一个 将使用特异性单抗进行循环检测。 在内皮损伤过程中释放的肺酶。

This proposal is to study mechanisms of immunological injury to vascular endothelium and to develop an assay for pulmonary endothelial injury. Human angiotensin converting enzyme, which is a constituent of the luminal membrane of endothelium, will be purified. The purified enzyme will be used to develop an enzyme-linked immunoassay to detect circulating antibodies in patients with systemic lupus erythematosus, progressive systemic sclerosis and adult respiratory distress syndrome. Polyclonal and monoclonal antibodies to angiotensin converting enzyme will be produced. These will be tested for their interaction with membrane bound enzyme in human endothelial cell culture. Platelet activating factor release and coagulant activity will be measured. Monoclonal antibodies will be tested for their specificity for pulmonary angiotensin converting enzyme. A specific monoclonal antibody will be used to assay for circulating pulmonary enzyme released during endothelial injury.