STRUCTURE AND FUNCTION OF TITIN IN CARDIAC MYOFIBRILS
STRUCTURE AND FUNCTION OF TITIN IN CARDIAC MYOFIBRILS
批准号:
3344511
负责人:
MARION Lewis GREASER
金额:
$6.34万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-07-01 至 1988-06-30
关键词:
affinity chromatography aging chemical structure function congestive heart failure density gradient ultracentrifugation gel electrophoresis heart contraction immunoelectron microscopy immunofluorescence technique monoclonal antibody muscle proteins myocardium myocardium disorder myofibrils nonmammalian vertebrate embryology sarcomeres striated muscles tissue /cell culture tissue /cell preparation
中文摘要
这项研究的目的是确定titin在结构中的作用
以及心肌和骨骼肌肌原纤维的功能。这种蛋白质,
据报道,它占骨骼的10%,占骨骼的18%
心肌肌原纤维,其亚基大小超过100万道尔顿。
目前正在进行研究,以确定Titin在脑内的位置
使用单抗检测肌原纤维。两种荧光标记都打开
完整和部分提取的肌原纤维和免疫电子显微镜
我们会进行研究。将尝试确定是否
粗丝或细丝与分离的肌动蛋白或其他特定的
蛋白质(如结蛋白、α-肌动蛋白、肌球蛋白、星云蛋白等)可能
互动。将进行研究以确定Titin的关系
肌原纤维形成的表现。骨骼中Titin的准确含量
在Percoll梯度上纯化的心肌原纤维将
被分析。Titin特性随年龄的变化将在
正常和心肌萎缩的仓鼠心脏。Titin的弹性性质
与心脏高静息张力之间的明显关系
肌肉和高含量的肌动蛋白表明它可能在
心脏功能。Titin的变化和/或损害很可能涉及到
心脏病发作后或充血性心脏发生的改变
失败了。
英文摘要
The purpose of this study is to define the role ot titin in the structure
and function of cardiac and skeletal muscle myofibrils. This protein,
which has been reported to constitute as much as 10% of skeletal and 18% of
cardiac myofibrils, has a subunit size in excess of 1,000,000 daltons.
Studies are currently in progress to define the location of titin in the
myofibril using monoclonal antibodies. Both fluorescence labeling on
intact and partially extracted myofibrils and immunoelectron microscopic
studies will be conducted. Attempts will be made to determine whether
thick or thin filaments bind to isolated titin or whether other specific
proteins (such as desmin, Alpha-actinin, myomesin, nebulin, etc.) may
interact. Studies will be conducted to determine the relationship of titin
appearance to myofibrillogenesis. The precise amount of titin in skeletal
and cardiac myofibrils which have been purified on Percoll gradients will
be analyzed. Changes in titin properties with age will be examined in
normal and cardiomyopatihic hamster hearts. The elastic nature of titin
and the apparent relationship between the high resting tension of cardiac
muscle and the high titin content suggests it may play a key role in
cardiac function. Changes and/or damage to titin may well be involved in
the alterations occurring after a heart attack or in congestive heart
failure.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
Regulation of binding of subfragment 1 in isolated rigor myofibrils.
分离的僵硬肌原纤维中亚片段 1 结合的调节。
DOI:
10.1083/jcb.111.6.2989
发表时间:
1990
期刊:
The Journal of cell biology
影响因子:
--
作者:
[Swartz,DR, Greaser,ML, Marsh,BB]
通讯作者:
Marsh,BB
Partial titin cDNA sequence isolated from rabbit cardiac muscle RNA.
从兔心肌 RNA 中分离出部分肌联蛋白 cDNA 序列。
DOI:
10.1007/bf00123100
发表时间:
1993
期刊:
Journal of muscle research and cell motility
影响因子:
2.7
作者:
[Fritz,JD, Wolff,JA, Greaser,ML]
通讯作者:
Greaser,ML
Developmental Changes Affecting Cardiac Titin Function
-
批准号:6892054
-
项目类别:
-
资助金额:$29.1万
-
财政年份:2004
-
负责人:MARION Lewis GREASER
-
依托单位:
Developmental Changes Affecting Cardiac Titin Function
-
批准号:7238617
-
项目类别:
-
资助金额:$27.59万
-
财政年份:2004
-
负责人:MARION Lewis GREASER
-
依托单位:
Titin Splicing Mechanisms and Physical Implications
-
批准号:7851384
-
项目类别:
-
资助金额:$36.31万
-
财政年份:2004
-
负责人:MARION Lewis GREASER
-
依托单位:
Titin Splicing Mechanisms and Physical Implications
-
批准号:7655665
-
项目类别:
-
资助金额:$41.0万
-
财政年份:2004
-
负责人:MARION Lewis GREASER
-
依托单位:
Developmental Changes Affecting Cardiac Titin Function
-
批准号:7057855
-
项目类别:
-
资助金额:$28.42万
-
财政年份:2004
-
负责人:MARION Lewis GREASER
-
依托单位:
Developmental Changes Affecting Cardiac Titin Function
-
批准号:6808924
-
项目类别:
-
资助金额:$29.1万
-
财政年份:2004
-
负责人:MARION Lewis GREASER
-
依托单位:
TITINS ROLE IN MODULATING CARDIAC PASSIVE TENSION
-
批准号:2835634
-
项目类别:
-
资助金额:$21.55万
-
财政年份:1999
-
负责人:MARION Lewis GREASER
-
依托单位:
TITINS ROLE IN MODULATING CARDIAC PASSIVE TENSION
-
批准号:6184796
-
项目类别:
-
资助金额:$19.22万
-
财政年份:1999
-
负责人:MARION Lewis GREASER
-
依托单位:
TITINS ROLE IN MODULATING CARDIAC PASSIVE TENSION
-
批准号:6537569
-
项目类别:
-
资助金额:$20.25万
-
财政年份:1999
-
负责人:MARION Lewis GREASER
-
依托单位:
TITINS ROLE IN MODULATING CARDIAC PASSIVE TENSION
-
批准号:6390322
-
项目类别:
-
资助金额:$19.73万
-
财政年份:1999
-
负责人:MARION Lewis GREASER
-
依托单位:
STRUCTURE AND FUNCTION OF TITIN IN CARDIAC MYOFIBRILS
-
批准号:3344510
-
项目类别:
-
资助金额:$6.73万
-
财政年份:1984
-
负责人:MARION Lewis GREASER
-
依托单位:
海外基金