BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
批准号:
3350398
负责人:
WILLIAM I ROSENBLUM
金额:
$23.0万
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-09-30 至 1995-09-29
关键词:
acetylcholine arterioles bradykinin brain circulation calcium channel blockers calcium transporting ATPase cardiovascular pharmacology cerebrovascular occlusions eicosanoid metabolism electron microscopy free radicals histamine laboratory mouse lipoxygenase microcirculation phorbols platelet aggregation prostacyclins prostaglandin inhibitors protein kinase C serotonin inhibitor thromboxanes vascular endothelium vasoconstriction vasoconstrictors vasodilators
中文摘要
我们的长期目标是扩大我们的知识有关的机制
调节大脑微循环 这些机制至关重要
为大脑提供适当的流量,其功能尤其是
对氧气和营养输送受损敏感。 本研究
关注内皮在控制小动脉直径中的作用,
内皮损伤会扰乱血流的方式。 两组
研究计划。 所有这些都是相对独特的,因为它们是在
对体内微循环的影响 小鼠大脑表面的小动脉
通过活体显微镜观察。 第一组研究调查了
内皮细胞在产生舒张或收缩因子中的作用
(EDRF,EDCF),当被选择性血管活性激动剂刺激时或在
血压升高的自动调节。 内皮损伤,
由氦氖激光/伊文思蓝原位产生的激光束引起聚焦损失,
生产EDRF或EDCF的能力,并提供了一种测试方法,
激动剂的作用依赖于这种内皮机制。
将使用药理学探针,然后进行化学测量,
确定内皮衍生介质的化学性质(例如,
前列腺素类、其它类花生酸类)。 我们还将使用药理学探针,
确定蛋白激酶C和鸟苷酸或腺苷酸的作用
环化酶和L-精氨酸介导的合成,释放或影响
内皮衍生介质。 第二组研究涉及
血管内皮损伤引起的血小板聚集
小动脉 药理学探针将用于研究改变的
抗聚集内皮衍生舒张因子的合成/释放
在由以下物质损伤的部位促进局部血小板粘附/聚集
光/染料。 在这样的部位捕获栓塞的血小板将是有利的。
研究了其与短暂性缺血性脑卒中的重复症状的相关性
攻击药理学探针将用于评估增加的
当流动时释放这种EDRF以减少血小板聚集,
剪切力增加。 L-精氨酸在调节细胞凋亡中的作用
这种EDRF的抗聚集和抗粘附性能将是
研究了
英文摘要
Our long term goals are to expand our knowledge concerning mechanisms
regulating brain microcirculation. These mechanisms are vitally important
in providing appropriate flow to the brain, whose function is especially
sensitive to impaired delivery of oxygen and nutrients. Present studies
focus on the role of endothelium in controlling diameter of arterioles, and
on the ways in which endothelial damage can derange flow. Two groups of
studies are planned. All are relatively unique because they are conducted
on microcirculation in vivo. Surface arterioles on the mouse brain are
observed by intravital microscopy. The first group of studies investigates
the role of endothelium in producing relaxing or constricting factors
(EDRFs, EDCFs) when stimulated by selective vasoactive agonists or during
autoregulation to an increase in blood pressure. Endothelial injury,
produced in situ by helium neon laser/Evans blue causes a focus of lost
capacity to produce EDRFs or EDCFs and provides a method for testing which
agonists are dependent upon such an endothelial mechanism for their action.
Pharmacologic probes, followed by chemical measurements will be used to
determine the chemical nature of the endothelium derived mediators (e.g.
prostanoids, other eicosanoids). We will also use pharmacologic probes to
determine the role of protein kinase C, and of guanylate or adenylate
cyclase and of L-arginine in mediating the synthesis, release or effects of
the endothelium derived mediators. The second group of studies concerns
the production of platelet aggregation by endothelial injury in the
arterioles. Pharmacologic probes will be used to study the role of altered
synthesis/release of an antiaggregant endothelium derived relaxing factor
in promoting local platelet adhesion/aggregation at a site injured by
light/dye. The capture of embolizing platelets at such a site will be
studied for its relevance to repetitive symptoms of transient ischemic
attacks. Pharmacologic probes will be used to assess the role of increased
release of such an EDRF in decreasing platelet aggregation when flow and
shear are increased. The role of L-arginine in regulating the
antiaggregating and antiadhesive properties of this EDRF will be
investigated.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
ROLE OF L-ARGININE IN CONTROL OF BRAIN MICROCIRCULATION
-
批准号:2222293
-
项目类别:
-
资助金额:$10.04万
-
财政年份:1991
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
ROLE OF L-ARGININE IN CONTROL OF BRAIN MICROCIRCULATION
-
批准号:3364681
-
项目类别:
-
资助金额:$9.57万
-
财政年份:1991
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
ROLE OF L-ARGININE IN CONTROL OF BRAIN MICROCIRCULATION
-
批准号:3364680
-
项目类别:
-
资助金额:$9.2万
-
财政年份:1991
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
-
批准号:2217972
-
项目类别:
-
资助金额:$26.14万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
-
批准号:3350401
-
项目类别:
-
资助金额:$19.88万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
-
批准号:3350404
-
项目类别:
-
资助金额:$25.05万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
-
批准号:3350399
-
项目类别:
-
资助金额:$19.97万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
-
批准号:3350402
-
项目类别:
-
资助金额:$21.15万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
-
批准号:3350405
-
项目类别:
-
资助金额:$26.03万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
-
批准号:3350403
-
项目类别:
-
资助金额:$23.18万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION AND ENDOTHELIAL INJURY
-
批准号:3350400
-
项目类别:
-
资助金额:$20.36万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCUATION AND ENDOTHELIAL INJURY
-
批准号:3350396
-
项目类别:
-
资助金额:$21.06万
-
财政年份:1985
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
BRAIN MICROCIRCULATION, PLATELETS, AND SEX HORMONES
-
批准号:3341437
-
项目类别:
-
资助金额:$14.7万
-
财政年份:1983
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
PLATELET AND MICROVASCULAR RESPONSES
-
批准号:3337279
-
项目类别:
-
资助金额:$8.11万
-
财政年份:1978
-
负责人:WILLIAM I ROSENBLUM
-
依托单位:
海外基金