ENDOCYTOSIS, CA2 AND MEMBRANE DEFECTS IN SICKLE CELLS

镰状细胞的内吞作用、CA2 和膜缺陷

• 批准号: 3353152
• 负责人: PATRICK WILLIAMSON
• 金额: $ 17.46万
• 依托单位: AMHERST COLLEGE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1989-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3353152
• 关键词: Fick method; calcium metabolism; cell adhesion; cytolysis; erythrocyte membrane; flow cytometry; fluorescence microscopy; fluorescent dye /probe; human subject; membrane activity; membrane lipids; membrane structure; receptor mediated endocytosis; reticulocytes; sickle cell anemia; sickle cell crisis; transferrin; vesicle /vacuole

Sickle cells contain small endocytic vesicles within which Ca++ is concentrated. Fluorescence assays which detect either the presence or the formation of these vesicles will be used to screen patient populations to determine whether vesicles are germane to the clinical course of the disease. Fick cytometry and fluorescence microscopy will be used to determine the characteristics and mechanism of vesicle formation, with particular attention to the question of the relationship between the endocytosis which produces these vesicles in sickle cells and classical receptor mediated endocytosis as it occurs in normal reticulocytes. Fluorescence assays will also be used to determine the conditions under which vesicle lysis and Ca++ release occurs. The characteristics of the Ca++ pulse which occurs when vesicles lyse will be measured. Using a variety of assays, several Ca++ sensitive red cell membrane characteristics, such as lipid asymmetry, adhesion, and ability to activate the coagulation mechanism, will be restudied to determine whether they are preferentially altered in cells in which vesicles occur, whether their sensitivity to Ca++ is such that they could be effected by a Ca++ pulse, and whether their sensitivity to Ca++ is enhanced in sickle cells.

镰状细胞含有小的内吞小泡，其中的钙离子是 集中精力。荧光分析可以检测到细菌的存在 这些囊泡的形成将被用于筛查患者群体 确定水泡是否与临床病程有关 疾病。Fick细胞术和荧光显微镜将用于 确定囊泡形成的特征和机制， 特别注意两国之间的关系 在镰状细胞和经典细胞中产生这些囊泡的内吞作用 在正常网织红细胞中发生的受体介导的内吞作用。 荧光分析也将被用来确定以下条件 发生囊泡溶解和钙离子释放。这些产品的特点 当囊泡溶解时发生的钙离子脉冲将被测量。使用 多种检测方法，几种钙离子敏感的红细胞膜 特征，如脂质不对称、粘连和激活能力 凝血机制，将被重新研究，以确定它们是否 在出现小泡的细胞中优先改变，无论它们的 对Ca++的敏感性使得它们可以受到Ca++脉冲的影响， 以及它们对钙离子的敏感性是否在镰状细胞中增强。