ENDOCYTOSIS, CA2 AND MEMBRANE DEFECTS IN SICKLE CELLS

镰状细胞含有小的内吞囊泡，其中Ca++被 集中 荧光测定法，其检测存在或 这些囊泡的形成将用于筛选患者群体， 确定囊泡是否与临床过程密切相关， 疾病 Fick细胞计数和荧光显微镜将用于 确定囊泡形成的特征和机制， 特别注意与人权的关系问题， 在镰状细胞中产生这些囊泡的内吞作用， 受体介导的内吞作用，如在正常网织红细胞中发生的那样。 还将使用荧光测定来确定在 发生囊泡溶解和Ca++释放。 的特点 将测量囊泡溶解时发生的Ca++脉冲。 使用 多种试验，几种Ca++敏感性红细胞膜 特征，如脂质不对称性，粘附性和激活能力 将重新研究凝血机制，以确定它们是否 优先在发生囊泡的细胞中改变，无论它们的 对Ca++的敏感性使得它们可以受到Ca++脉冲的影响， 以及镰状细胞对Ca++的敏感性是否增强。

Sickle cells contain small endocytic vesicles within which Ca++ is concentrated. Fluorescence assays which detect either the presence or the formation of these vesicles will be used to screen patient populations to determine whether vesicles are germane to the clinical course of the disease. Fick cytometry and fluorescence microscopy will be used to determine the characteristics and mechanism of vesicle formation, with particular attention to the question of the relationship between the endocytosis which produces these vesicles in sickle cells and classical receptor mediated endocytosis as it occurs in normal reticulocytes. Fluorescence assays will also be used to determine the conditions under which vesicle lysis and Ca++ release occurs. The characteristics of the Ca++ pulse which occurs when vesicles lyse will be measured. Using a variety of assays, several Ca++ sensitive red cell membrane characteristics, such as lipid asymmetry, adhesion, and ability to activate the coagulation mechanism, will be restudied to determine whether they are preferentially altered in cells in which vesicles occur, whether their sensitivity to Ca++ is such that they could be effected by a Ca++ pulse, and whether their sensitivity to Ca++ is enhanced in sickle cells.