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ENDOCYTOSIS, CA2 AND MEMBRANE DEFECTS IN SICKLE CELLS

镰状细胞的内吞作用、CA2 和膜缺陷

基本信息

批准号：
3353156
负责人：
PATRICK WILLIAMSON
金额：
$ 19.22万
依托单位：
AMHERST COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-09-30 至 1990-09-29
项目状态：
已结题

项目摘要

Sickle cells contain small endocytic vesicles within which Ca++ is concentrated. Fluorescence assays which detect either the presence or the formation of these vesicles will be used to screen patient populations to determine whether vesicles are germane to the clinical course of the disease. Fick cytometry and fluorescence microscopy will be used to determine the characteristics and mechanism of vesicle formation, with particular attention to the question of the relationship between the endocytosis which produces these vesicles in sickle cells and classical receptor mediated endocytosis as it occurs in normal reticulocytes. Fluorescence assays will also be used to determine the conditions under which vesicle lysis and Ca++ release occurs. The characteristics of the Ca++ pulse which occurs when vesicles lyse will be measured. Using a variety of assays, several Ca++ sensitive red cell membrane characteristics, such as lipid asymmetry, adhesion, and ability to activate the coagulation mechanism, will be restudied to determine whether they are preferentially altered in cells in which vesicles occur, whether their sensitivity to Ca++ is such that they could be effected by a Ca++ pulse, and whether their sensitivity to Ca++ is enhanced in sickle cells.

镰状细胞含有小的内吞囊泡，其中Ca++被集中荧光测定法，其检测存在或这些囊泡的形成将用于筛查患者群体，确定囊泡是否与临床过程密切相关，疾病 Fick细胞计数和荧光显微镜将用于确定囊泡形成的特征和机制，特别注意与人权的关系问题，在镰状细胞中产生这些囊泡的内吞作用，受体介导的内吞作用，如在正常网织红细胞中发生的那样。还将使用荧光测定来确定在发生囊泡溶解和Ca++释放。的特点将测量囊泡溶解时发生的Ca++脉冲。使用多种试验，几种Ca++敏感性红细胞膜特征，如脂质不对称性，粘附性和激活能力将重新研究凝固机制，以确定它们是否优先在发生囊泡的细胞中改变，无论它们的对Ca++的敏感性使得它们可以受到Ca++脉冲的影响，以及镰状细胞对Ca++的敏感性是否增强。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Peroxidation-induced perturbations of erythrocyte lipid organization.

过氧化诱导的红细胞脂质组织扰动。

DOI：
10.1016/0005-2736(90)90132-8
发表时间：
1990
期刊：
Biochimica et biophysica acta
影响因子：
0
作者：
Pradhan,D;Weiser,M;Lumley-Sapanski,K;Frazier,D;Kemper,S;Williamson,P;Schlegel,RA
通讯作者：
Schlegel,RA

Ca2+ accumulation and loss by aberrant endocytic vesicles in sickle erythrocytes.

镰状红细胞中异常内吞囊泡的 Ca2 积累和损失。