IMMUNE FUNCTIONS OF ACCESSORY CELLS IN THE LUNG

本研究的目的是分析小鼠的免疫功能。 肺中的辅助细胞及其与免疫反应的关系 由于吸入病原体和外来抗原而触发。附件 在大多数免疫反应的诱导过程中，细胞必须表达MHC 第二类抗原(Ia)在其表面，并具有内吞能力， 处理抗原并将其呈递给T细胞。 其具体目的是鉴定Lewis大鼠肺中的那些细胞 有提出抗原和分析缺陷的能力(S) 使肺泡巨噬细胞(AM)不能有效地提呈抗原 T细胞。这将通过使用天然鸡蛋溶菌酶来实现。 (HEL)和包含主要T细胞表位的多肽片段。这个 提呈抗原的能力将通过免疫T细胞来测量 增殖试验。双标记免疫凝胶技术将使用HIGH 兔抗FITC抗体与鼠抗鼠Ia的亲和力 用于识别FITC-HEL通过的细胞室的抗体 在细胞表面呈现之前通过并确定它是否 在单元内的任何时间变为与Ia相关联。它的本质就是 将检测AM表面T细胞的假定结合活性。 细胞分离技术将用于分离间质巨噬细胞， 树突状细胞和肺泡II型肺泡细胞检测其能力 表达Ia或在受到刺激后表达 富含干扰素的淋巴因子。他们将接受检查，看他们是否有能力 HEL抗原呈递给HEL免疫T细胞并表达白介素I 活动。最后，这些细胞与免疫T细胞的相互作用将 在HEL免疫的Lewis大鼠中被检测到 共价连接到15 nm金上的可溶性或不溶性HEL的给药 粒子。 这些研究的结果应该会让我们更好地理解 AM所经历的调整导致他们未能 有效地提呈抗原。他们还将识别这些细胞在 肺作为合格的抗原提呈细胞。

The objective of our research is to analyze the immune function of accessory cells in the lung and to relate these to immune responses triggered as a result of inhaled pathogens and foreign antigens. Accessory cells, during the induction of most immune responses, must express MHC class II antigens (Ia) on their surface and have the ability to endocytose, process and present antigen to T-cells. The specific aims are to identify those cells in the Lewis rat lung which have the ability to present antigen ad to analyze the defect(s) which render the alveolar macrophage (AM) unable to effectively present antigen to T-cells. This will be accomplished by using native hen egg lysozyme (HEL) and peptide fragments containing the major T-cell epitope. The ability to present antigen will be measured by means of an immune T-cell proliferation assay. Double labelling immunogols techniques will use high affinity rabbit anti-FITC antibodies and monoclonal mouse anti-rat Ia antibodies to identify the cellular compartments through which FITC-HEL passes before presentation on the cell surface and to establish whether it becomes associated with Ia at any time within the cell. The nature of a putative binding activity for T cells on the AM surface will be examined. Cell isolation techniques will be used to isolate interstitial macrophages, dendritic cells, and alveolar type II pneumocytes to test for their ability to either express Ia constituitively or following stimulation with interferon-enriched lymphokine. They will be examined for their ability to present HEL antigen to HEL immune T-cells and to express interleukin I activity. Finally the interaction of these cells with immune T-cells will be examined in HEL-immunized Lewis rats challenged by the intratrached administration of soluble or insoluble HEL covalently linked to 15 nm gold particles. Results from these studies should lead to a better understanding of the adaptations AM have undergone that have resulted in their failure to present antigen effectively. They will also identify those cells in the lung which serve as competent antigen presenting cells.