BARORECPTOR MORPHOLOGY DURING HYPERTENSION

该项目的具体目标是检验以下假设： 原发性高血压期间的压力感受器重置部分是由于 颈动脉的神经支配和/或血液供应的形态学变化 窦压力感受器区域本身。 这些具体目标将在 通过实验确定：（1）高血压是否会导致 鼻窦血液供应的变化;（2）如果有形态学 窦壁的改变可以解释 该血管区的扩张性;（3）高血压是否开始或 受压力感受器和交感神经的增加或减少的影响 纤维神经支配的窦;和（4）如果改变的形态， 压力感受器神经末梢和与它们耦合的组织发生 高血压之前或高血压的结果 血管铸型/扫描 电镜，亚甲蓝活体染色，选择性神经 变性和定量透射电子显微镜技术 将被用来回答有关形态学的重要问题， 关键的压力感受区。 的 自发性高血压大鼠（SHR）及其正常血压Wistar-Kyoto (WKY)染色将用作人类原发性高血压模型， 这些研究。 对基本的压力感受器知之甚少， 交感神经支配和颈动脉窦的血液供应。 研究 在本申请中概述的将提供非常需要的信息， 客观地获得，关于压力感受器的形态学相关性， 参与早期和进行性原发性高血压，病因 其中有哪些仍然未知。

The specific aims of this project are to test the hypothesis that baroreceptor resetting during essential hypertension is due, in part, to a morphological change in the innervation and/or blood supply to the carotid sinus baroreceptor region itself. These specific aims will be approached experimentally by determining: (1) whether hypertension precipitates changes in the blood supply to the sinus; (2) if there are morphological alterations to the sinus wall which would account for decreased distensibility of this vascular zone; (3) whether hypertension initiates or is affected by an increase or decrease in baroreceptor and sympathetic fiber innervation to the sinus; and (4) if alterations in the morphology of baroreceptor nerve terminals and the tissue they are coupled with occur before, or as a result of, hypertension. Vascular casting/scanning electron microscopy, methylene blue vital staining, selective nerve degeneration, and quantitative transmission electron microscopy techniques will be used to answer important questions about the morphology of this crucial baroreceptive zone during progressive hypertension. The spontaneously hypertensive rat (SHR) and its normotensive Wistar-Kyoto (WKY) stain will be used as a model of human essential hypertension in these studies. Little is known about the basic baroreceptor and sympathetic innervation and blood supply to the carotid sinus. The studies outlined in this application will provide much needed information, objectively obtained, about the morphological correlates of baroreceptor involvement in early and progressive essential hypertension, the etiology of which remains unknown.