HEART-SPECIFIC CREATINE KINASE REGULATORY ELEMENTS
HEART-SPECIFIC CREATINE KINASE REGULATORY ELEMENTS
批准号:
3355618
负责人:
STEPHEN DENISON HAUSCHKA
金额:
$8.3万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-08-01 至 1990-07-31
关键词:
DNA biochemical evolution cell type clone cells cow creatine kinase developmental genetics embryo /fetus genetic promoter element goats heart cell heart function heart metabolism horses laboratory mouse laboratory rabbit molecular cloning muscle cells phosphates regulatory gene smooth muscle striated muscles transfection ventricular hypertrophy
中文摘要
本提案的总体目标是确定如何
英文摘要
The overall goal of this proposal is to determine how the
expression of two genetic isoforms of creatine kinase (MCK and
BCK) is regulated in mammalian heart cells. The specific
objectives are: (1) to identify cis-acting DNA elements within the
mouse MCK gene which are required for tissue- and/or cardiac
cell type-specific expression of MCK; (2) to identify cis-acting
DNA elements within the MCK gene which are required for
various physiological modulations of its expression; (3) to clone
the 5'-flanking and first intron portion of the mouse BCK gene,
and to identity similar cell type and physiological regulatory
elements within it; (4) to identify and begin purification of the
trans-acting factors that bind to these DNA regions or that
selectively stimulate or repress transcription from the M- and
BCK promoter regions; and (5) to derive permanent cell lines
which are representative of the various cardiac muscle cell types
(atrial, ventricular, and conducting), as well as cardiac progenitor
cells.
Basic knowledge derived from these studies may be particularly
informative with respect to the general problem of tissue-specific
gene regulation during development. Our approach will permit
direct comparisons between how the expression of two
evolutionarily-related genes is regulated within a set of
developmentally-related cell types in the cardiac and skeletal
muscle cell lineages. In addition, since the creatine kinases play a
critical role in the high-energy phosphate shuttle of cardiac
muscle cells, and since CK levels appear to change in response to
cardiac hypertrophy, these studies should provide information
which is relevant to general problems of heart disease. Finally,
the availability of permanent cell lines representing different
cardiac muscle cell types would be of great value to virtually all
studies of heart cell function.
Major methodologies include: gene cloning, modification,
transfection, DNAase foot-printing, gel retardation, in vitro
transcription assays, clonal cell culture, and spontaneous,
oncogene-, and viral-induced transformation.
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Development of high activity human muscle-specific regulatory cassettes and their
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批准号:8378057
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项目类别:
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资助金额:$27.83万
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财政年份:2004
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负责人:STEPHEN DENISON HAUSCHKA
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依托单位:
Cell Culture Models for Testing Dystrophobic Muscle Gene Therapy
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批准号:6803771
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项目类别:
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资助金额:$28.85万
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财政年份:2004
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负责人:STEPHEN DENISON HAUSCHKA
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依托单位:
Development of high activity human muscle-specific regulatory cassettes and their
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批准号:8048042
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项目类别:
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资助金额:$29.21万
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财政年份:2004
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负责人:STEPHEN DENISON HAUSCHKA
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依托单位:
Development of high activity human muscle-specific regulatory cassettes and their
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批准号:7664780
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项目类别:
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资助金额:$28.65万
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财政年份:2004
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负责人:STEPHEN DENISON HAUSCHKA
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依托单位:
Development of high activity human muscle-specific regulatory cassettes and their
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批准号:8447008
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项目类别:
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资助金额:$26.19万
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财政年份:2004
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负责人:STEPHEN DENISON HAUSCHKA
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依托单位:
Development of high activity human muscle-specific regulatory cassettes and their
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批准号:8233484
-
项目类别:
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资助金额:$29.44万
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财政年份:2004
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负责人:STEPHEN DENISON HAUSCHKA
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依托单位:
HEART-SPECIFIC CREATINE KINASE REGULATORY ELEMENTS
-
批准号:3355615
-
项目类别:
-
资助金额:$8.25万
-
财政年份:1987
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
CREATINE KINASE CONTROL ELEMENTS & CARDIAC DETERMINATION
-
批准号:3355620
-
项目类别:
-
资助金额:$19.7万
-
财政年份:1987
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
CREATINE KINASE CONTROL ELEMENTS & CARDIAC DETERMINATION
-
批准号:3355619
-
项目类别:
-
资助金额:$18.34万
-
财政年份:1987
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
HEART-SPECIFIC CREATINE KINASE REGULATORY ELEMENTS
-
批准号:3355617
-
项目类别:
-
资助金额:$8.01万
-
财政年份:1987
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
CREATINE KINASE CONTROL ELEMENTS & CARDIAC DETERMINATION
-
批准号:3355616
-
项目类别:
-
资助金额:$10.36万
-
财政年份:1987
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
Muscle Gene Regulation and Cassettes for Gene Therapy
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批准号:6578352
-
项目类别:
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资助金额:$35.31万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
Muscle Gene Regulation and Cassettes for Gene Therapy
-
批准号:7257075
-
项目类别:
-
资助金额:$30.77万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
Striated Muscle Gene Transcription and Regulatory Gene Cassettes for Gene Therapy
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批准号:8277091
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项目类别:
-
资助金额:$32.62万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
Striated Muscle Gene Transcription and Regulatory Gene Cassettes for Gene Therapy
-
批准号:7858353
-
项目类别:
-
资助金额:$33.98万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
REGULATION OF SKELETAL MUSCLE DIFFERENTIATION
-
批准号:2078397
-
项目类别:
-
资助金额:$26.35万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
REGULATION OF SKELETAL MUSCLE DIFFERENTIATION
-
批准号:2078398
-
项目类别:
-
资助金额:$27.94万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
MITOGENIC REGULATION OF SKELETAL MUSCLE DIFFERENTIATION
-
批准号:3154998
-
项目类别:
-
资助金额:$13.64万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
MITOGENIC REGULATION OF SKELETAL MUSCLE DIFFERENTIATION
-
批准号:3151167
-
项目类别:
-
资助金额:$16.3万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
Muscle Gene Regulation and Cassettes for Gene Therapy
-
批准号:7064253
-
项目类别:
-
资助金额:$31.7万
-
财政年份:1976
-
负责人:STEPHEN DENISON HAUSCHKA
-
依托单位:
海外基金