BIOCHEMICAL EVOLUTION OF ISCHEMIC BRAIN DAMAGE
BIOCHEMICAL EVOLUTION OF ISCHEMIC BRAIN DAMAGE
批准号:
3396867
负责人:
FRANK A WELSH
金额:
$10.47万
依托单位国家:
美国
项目类别:
财政年份:
1980
资助国家:
美国
项目状态:
已结题
起止时间:
1980-07-01 至 1986-06-30
中文摘要
本研究的目的是确定是否组织酸中毒
英文摘要
The goal of this investigation is to determine whether tissue acidosis
plays a critical role in the development of ischemic damage and, if so, by
which biochemical mechanisms. Using a model of reversible, unilateral
hypoxia-ischemia in the mouse, we will first develop a regional,
quantitative method for brain pH. Second, ischemic alterations of brain pH
will be correlated with the accumulation of lactate during hypoxia-ischemia
and with postischemic recovery of energy metabolism. Third, the pH changes
during hypoxia-ischemia will be modified (a) by administering CO2 and (b)
by partially inhibiting the glycolytic pathway in order to alter the
recovery of energy metabolism. Finally, the ischemic change in glycolytic
intermediates other than lactate will be determined in glucose-pretreated
animals to detect deleterious alterations not related to acidosis. The
second major aim is to identify the biochemical processes which are
affected by acidosis/glucose-pretreatment. Because NADH is acid-labile, we
will measure brain levels of NADH, NAD+, and their degradation products
using enzymatic methods and high performance liquid chromatography (HPLC).
In addition, we will attempt to alter the size of the NAD pool by
administering the precursor, nicotinamide, and a false precursor,
6-amino-nicotinamide. The effect of acidosis/glucose-pretreatment on brain
levels of Adenyl, Guanyl, Uridyl, and Cytidyl Nucleotides during and after
hypoxia-ischemia will be determined using HPLC. Finally, the degradative
activity of acid phosphatase will be assayed in hypoxic-ischemic brain
using both in vitro and in vivo methods.
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CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
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批准号:6539715
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项目类别:
-
资助金额:$27.74万
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财政年份:1991
-
负责人:FRANK A WELSH
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依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
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批准号:2267522
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项目类别:
-
资助金额:$15.75万
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财政年份:1991
-
负责人:FRANK A WELSH
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依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
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批准号:2891795
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项目类别:
-
资助金额:$31.7万
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财政年份:1991
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负责人:FRANK A WELSH
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依托单位:
Cerebral ischemia and expression of stress genes
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批准号:7740782
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项目类别:
-
资助金额:$34.11万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
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批准号:2702992
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项目类别:
-
资助金额:$30.48万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:2267524
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项目类别:
-
资助金额:$28.18万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:6639427
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项目类别:
-
资助金额:$27.74万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
Cerebral ischemia and expression of stress genes
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批准号:7197449
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项目类别:
-
资助金额:$34.14万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:2267523
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项目类别:
-
资助金额:$27.55万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:3416111
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项目类别:
-
资助金额:$14.72万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:3416110
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项目类别:
-
资助金额:$14.44万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:6741943
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项目类别:
-
资助金额:$27.74万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:2416299
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项目类别:
-
资助金额:$29.31万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:3416112
-
项目类别:
-
资助金额:$14.88万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
CEREBRAL ISCHEMIA AND EXPRESSION OF STRESS GENES
-
批准号:6331156
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项目类别:
-
资助金额:$27.74万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
Cerebral ischemia and expression of stress genes
-
批准号:7534983
-
项目类别:
-
资助金额:$34.45万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
Cerebral ischemia and expression of stress genes
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批准号:7342510
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项目类别:
-
资助金额:$34.45万
-
财政年份:1991
-
负责人:FRANK A WELSH
-
依托单位:
BIOCHEMICAL EVOLUTION OF ISCHEMIC BRAIN DAMAGE
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批准号:3396868
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项目类别:
-
资助金额:$13.92万
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财政年份:1980
-
负责人:FRANK A WELSH
-
依托单位:
BIOCHEMICAL EVOLUTION OF ISCHEMIC BRAIN DAMAGE
-
批准号:3396869
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项目类别:
-
资助金额:$13.03万
-
财政年份:1980
-
负责人:FRANK A WELSH
-
依托单位:
BIOCHEMICAL EVOLUTION OF ISCHEMIC BRAIN DAMAGE
-
批准号:3396863
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项目类别:
-
资助金额:$13.15万
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财政年份:1980
-
负责人:FRANK A WELSH
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依托单位:
国内基金
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肿瘤微环境因子Lactic acidosis在肿瘤细胞耐受葡萄糖剥夺中的作用机制研究
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批准号:81301707
-
项目类别:青年科学基金项目
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资助金额:23.0万元
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批准年份:2013
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负责人:吴昊
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依托单位: