CARNITINE TRANSPORT IN NATURAL AND ARTIFICIAL MEMBRANE

天然和人造膜中肉碱的转运

基本信息

项目摘要

The long term objectives of this proposal are to elucidate the mechanism of acylcarnitine translocation across the mitochondrial inner membrane and to determine factors which interact with the translocase and control acylcarnitine cellular distribution. The overall specific aims are to determine structural and functional properties of the translocase isolated from beef heart submitochondrial particles. The translocase will be purified on a carnitine affinity column and activity reconstituted into phospholipid vesicles. Physical characterization of the isolated protein(s) will include: molecular weight, subunit composition, inhibitor-binding peptides, stoichiometry and affinity of substrate sites; as well as the transbilayer orientation in the presence or absence of substrate using the hydrophilic surface probe, 3-Azido-2,7-Naphthalene Disulfonate. Exchange and/or unidirectional transport activities will be determined. Reconstitution of the translocase will be otpimezed for phospholipid class and acyl chain saturation. The effect of the translocase on intervesicular transfer of acylcarnitine and interaction with fatty acid binding protein will be determined using the concentration sensitive probe, pyrenedodecanoyl-carnitine. Finally, the functional relationship of the translocase with purified carnitine palmitoyltransferase will be determined in phospholipid vesicles by fluorescent energy transfer techniques and by determination of [14C] carnitine release from translocated palmitoyl[14C]carnitine. Studies on the purified translocase are relevant to disease-associated impairment in either the translocase or translocase/transferase coupling, e.g., lipid storage myopathies, muscle dystrophy and certain cardiomyopathies.
这一建议的长期目标是阐明机制

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Jeanie B. MC MILLIN其他文献

Jeanie B. MC MILLIN的其他文献

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{{ truncateString('Jeanie B. MC MILLIN', 18)}}的其他基金

LOW DOSE ALCOHOL--ATTENUATION OF CARDIAC APOPTOSIS
低剂量酒精--心肌细胞凋亡的减弱
  • 批准号:
    2880981
  • 财政年份:
    1999
  • 资助金额:
    $ 9.41万
  • 项目类别:
LOW DOSE ALCOHOL--ATTENUATION OF CARDIAC APOPTOSIS
低剂量酒精--心肌细胞凋亡的减弱
  • 批准号:
    6168501
  • 财政年份:
    1999
  • 资助金额:
    $ 9.41万
  • 项目类别:
FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
心肌缺血时的脂肪酸代谢
  • 批准号:
    2219083
  • 财政年份:
    1989
  • 资助金额:
    $ 9.41万
  • 项目类别:
FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
心肌缺血中的脂肪酸代谢
  • 批准号:
    3355300
  • 财政年份:
    1989
  • 资助金额:
    $ 9.41万
  • 项目类别:
FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
心肌缺血时的脂肪酸代谢
  • 批准号:
    2745631
  • 财政年份:
    1989
  • 资助金额:
    $ 9.41万
  • 项目类别:
FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
心肌缺血中的脂肪酸代谢
  • 批准号:
    3355301
  • 财政年份:
    1989
  • 资助金额:
    $ 9.41万
  • 项目类别:
FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
心肌缺血中的脂肪酸代谢
  • 批准号:
    6330031
  • 财政年份:
    1989
  • 资助金额:
    $ 9.41万
  • 项目类别:
FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
心肌缺血时的脂肪酸代谢
  • 批准号:
    2219084
  • 财政年份:
    1989
  • 资助金额:
    $ 9.41万
  • 项目类别:
FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
心肌缺血中的脂肪酸代谢
  • 批准号:
    3355297
  • 财政年份:
    1989
  • 资助金额:
    $ 9.41万
  • 项目类别:
FATTY ACID METABOLISM IN CARDIAC ISCHEMIA
心肌缺血时的脂肪酸代谢
  • 批准号:
    6125761
  • 财政年份:
    1989
  • 资助金额:
    $ 9.41万
  • 项目类别:

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