THROMBOSIS ON BIOMATERIALS:ROLE OF VASCULAR COMPONENTS
生物材料上的血栓形成:血管成分的作用
基本信息
- 批准号:3355421
- 负责人:
- 金额:$ 27.36万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1987
- 资助国家:美国
- 起止时间:1987-09-30 至 1994-09-29
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The problem of limited availability of biological replacements for
diseased vessels and organs is exacerbated by the failure of most
prosthetic implants and organ substitutes due to repeated
thrombo-embolic events. The mechanism which underlie the
deposition of thrombotic masses on biomaterial surfaces is poorly
understood. The objective of the present proposal is to
investigate the influence of vascular surfaces on the deposition of
platelets and fibrin on selected prosthetic surfaces. A tubular
perfusion chamber has been developed for mounting the materials
immediately downstream of injured vascular surfaces exposing the
composite substrates to blood in an ex vivo perfusion system.
Blood from both swine and human subjects will be utilized to
investigate the specific mechanisms which are important in
determining the deposition of thrombotic material on the
biomaterial surface. Evaluation of the surface deposits will be
performed by several techniques. In swine, III-In labelled
platelets will be injected and the quantity of deposited
radioactivity determined. In swine and humans, morphometric
analysis of epoxy embedded semi-thin sections will be utilized to
quantify platelet and fibrin deposits. The thrombotic deposits will
be evaluated for several exposure times, axial positions from the
proximal surface and wall shear rates, such that the full
physiological range of flow conditions is investigated (wall shear
rates from 50-5000 sec-1).
Variation of proximal (vascular) and distal (biomaterial) surfaces
and the condition of the blood will utilized to explore the
mechanisms of platelet and fibrin deposition on the prosthetic
surface. Various components of the vessel wall, including,
subendothelium and its modifications, collagen, and tissue factor
will be utilized as the activating proximal surface. Blood from
patients and animal models with various bleeding disorders will be
utilized to investigate the importance of von Willebrand factor,
platelet glycoproteins Ib and IIb-IIIa, platelet released products
and the relative importance of the Factor XI, coagulation and the
tissue factor, Factor VII, IX pathways. Antibodies of VWF, and
glycoproteins Ib and IIb-IIIa will also be employed and compared
to results obtained with the corresponding naturally occurring
deficiency.
The mechanisms of growth of thrombotic deposits will also be
investigated in nonstreamline (recirculation) flow by appropriately
modifying the perfusion chambers and a theory for particle
deposition will be developed.
生物替代品有限的问题,
大多数血管和器官的功能衰竭会加剧病变。
假体植入物和器官替代品,
血栓栓塞事件。 其背后的机制
血栓块在生物材料表面上的沉积
明白 本建议的目的是
研究血管表面对沉积的影响,
血小板和纤维蛋白。 管状
灌注室已被开发用于安装材料
紧接着受损血管表面的下游,
在离体灌注系统中将复合基质与血液混合。
将使用猪和人类受试者的血液,
研究重要的具体机制,
测定血栓物质在血管上的沉积,
生物材料表面 表面沉积物的评估将
通过几种技术进行。 在猪中,标记III-In
将注射血小板,并确定沉积的血小板数量。
放射性测定。 在猪和人类中,形态测量
将利用环氧树脂嵌入半薄切片的分析,
定量血小板和纤维蛋白沉积。 血栓沉积物将
对几个暴露时间、从
近端表面和壁剪切速率,使得
研究了流动条件的生理范围(壁面剪切
速率从50-5000秒-1)。
近端(血管)和远端(生物材料)表面的变化
血液的状况将被用来探索
血小板和纤维蛋白在假体上沉积的机制
面 血管壁的各种成分,包括,
内皮下及其修饰、胶原和组织因子
将用作激活近端表面。 血液从
患有各种出血性疾病的患者和动物模型将被
用于研究血管性血友病因子的重要性,
血小板糖蛋白Ib和IIb-IIIa,血小板释放产物
以及凝血因子XI、凝血和
组织因子、因子VII、IX途径。 VWF抗体,和
糖蛋白Ib和IIb-IIIa也将被采用和比较
与用相应的天然存在的
缺陷
血栓沉积物生长的机制也将被讨论。
在非流线(再循环)流动中,
修改灌注室和粒子理论
沉积将被开发。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The Effect of Flow on Hemostasis and Thrombosis
- DOI:10.1055/s-0038-1646406
- 发表时间:1991-09
- 期刊:
- 影响因子:6.7
- 作者:Y. Nemerson;V. Turitto
- 通讯作者:Y. Nemerson;V. Turitto
Further Evidence that Glycoprotein IIb-IIIa Mediates Platelet Spreading on Subendothelium
糖蛋白 IIb-IIIa 介导血小板在内皮下扩散的进一步证据
- DOI:
- 发表时间:1991
- 期刊:
- 影响因子:6.7
- 作者:H. Weiss;V. Turitto;H. Baumgartner
- 通讯作者:H. Baumgartner
Inhibition of growth of thrombus on fresh mural thrombus. Targeting optimal therapy.
- DOI:10.1161/01.cir.90.5.2432
- 发表时间:1994-11
- 期刊:
- 影响因子:37.8
- 作者:Beat J. Meyer;J. Badimón;A. Mailhac;Antonio Fernández-Ortiz;J. Chesebro;V. Fuster;L. Badimón
- 通讯作者:Beat J. Meyer;J. Badimón;A. Mailhac;Antonio Fernández-Ortiz;J. Chesebro;V. Fuster;L. Badimón
Use of enzyme-linked immunosorbent assay (ELISA) for detection and quantification of monoclonal antibodies.
使用酶联免疫吸附测定 (ELISA) 检测和定量单克隆抗体。
- DOI:
- 发表时间:1994
- 期刊:
- 影响因子:1
- 作者:Lewis,G
- 通讯作者:Lewis,G
Synergistic action of severe wall injury and shear forces on thrombus formation in arterial stenosis: definition of a thrombotic shear rate threshold.
严重壁损伤和剪切力对动脉狭窄血栓形成的协同作用:血栓剪切率阈值的定义。
- DOI:10.1016/0735-1097(94)90875-3
- 发表时间:1994
- 期刊:
- 影响因子:24
- 作者:Merino,A;Cohen,M;Badimon,JJ;Fuster,V;Badimon,L
- 通讯作者:Badimon,L
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VINCENT T. TURITTO其他文献
VINCENT T. TURITTO的其他文献
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{{ truncateString('VINCENT T. TURITTO', 18)}}的其他基金
TISSUE FACTOR ACTIVATION OF FACTOR X MEDIATED BY FLOW
血流介导的组织因子 X 因子激活
- 批准号:
2667416 - 财政年份:1998
- 资助金额:
$ 27.36万 - 项目类别:
BLOOD FLOW IN TISSUE FACTOR MEDIATED COAGULATION
组织因子介导的凝血中的血流
- 批准号:
6241767 - 财政年份:1997
- 资助金额:
$ 27.36万 - 项目类别:
THROMBOSIS ON BIOMATERIALS:ROLE OF VASCULAR COMPONENTS
生物材料上的血栓形成:血管成分的作用
- 批准号:
3355420 - 财政年份:1987
- 资助金额:
$ 27.36万 - 项目类别:
THROMBOSIS ON BIOMATERIALS--VASCULAR COMPONENTS
生物材料上的血栓形成——血管成分
- 批准号:
3355419 - 财政年份:1987
- 资助金额:
$ 27.36万 - 项目类别:
THROMBOSIS ON BIOMATERIALS--VASCULAR COMPONENTS
生物材料上的血栓形成——血管成分
- 批准号:
3355418 - 财政年份:1987
- 资助金额:
$ 27.36万 - 项目类别:
THROMBOSIS ON BIOMATERIALS:ROLE OF VASCULAR COMPONENTS
生物材料上的血栓形成:血管成分的作用
- 批准号:
3355417 - 财政年份:1987
- 资助金额:
$ 27.36万 - 项目类别:
THROMBOSIS ON BIOMATERIALS--VASCULAR COMPONENTS
生物材料上的血栓形成——血管成分
- 批准号:
3355414 - 财政年份:1987
- 资助金额:
$ 27.36万 - 项目类别:
ROLE OF BLOOD/VESSEL WALL COMPONENTS IN THROMBOSIS
血液/血管壁成分在血栓形成中的作用
- 批准号:
3348677 - 财政年份:1985
- 资助金额:
$ 27.36万 - 项目类别: