Further Evidence that Glycoprotein IIb-IIIa Mediates Platelet Spreading on Subendothelium
Further Evidence that Glycoprotein IIb-IIIa Mediates Platelet Spreading on Subendothelium
复制标题
糖蛋白 IIb-IIIa 介导血小板在内皮下扩散的进一步证据
DOI：
--
复制
发表时间：
1991
影响因子：
6.7
通讯作者：
H. Baumgartner
中科院分区：
文献类型：
--
作者：
H. Weiss;V. Turitto;H. Baumgartner
为了进一步探索糖蛋白GPIIb-IIIa促进血小板血管壁相互作用的机制，在环形室中研究了血小板与内皮下的粘附，其中将兔主动脉的内皮下以2，600 s−1的剪切速率暴露于血栓无力症患者的血液中。在5个暴露时间（1、2、3、5和10分钟）中的每一个时间进行灌注，并测定在接触（C）和扩散（S）阶段中具有血小板的血管段的表面覆盖百分比。在所有暴露时间，血小板无力症患者的血小板接触（C）值均增加;根据先前描述的血小板粘附于内皮下的动力学模型，这一发现与血小板增殖缺陷一致。根据这种附着模型，在单次暴露时间内血小板接触（C）值的增加可能表明扩散缺陷（S）或初始接触（C），但多次暴露仅会导致与血小板扩散缺陷相关的缺陷接触增加。在广泛的暴露时间范围内获得的结果提供了更确凿的证据表明，GPIIb-IIIa介导的血小板扩散比以前获得的单次暴露时间。
Summary In order to explore further the mechanism by which glycoprotein GPIIb-IIIa promotes platelet vessel wall interaction, platelet adhesion to subendothelium was studied in an annular chamber in which subendothelium from rabbit aorta was exposed at a shear rate of 2,600 s−1 to blood from patients with thrombasthenia. Perfusions were conducted for each of 5 exposure times (1 ,2,3, 5 and 10 min), and the percent surface coverage of the vessel segment with platelets in the contact (C) and spread (S) stage was determined. Increased values of platelet contact (C) were obtained in thrombasthenia at all exposure times; this finding is consistent with a defect in platelet spreadirg, based on a previously described kinetic model of platelet attachment to subendothelium. According to this model of attachment, increased values of platelet contact (C) at a single exposure time may be indicative of either a defect in spreading (S) or initial contact (C), but multiple exposures will result in increased contact only for defects which are related to defectiye platelet spreading (s). The results obtained over a broad range of exposure times provide more conclusive evidence that GPIIb-IIIa mediates platelet spreading than those previously obtained at single exposure times.
登录
查看更多内容
来自血小板、单核细胞和内皮的生长因子：它们在细胞增殖中的作用。
影响因子：
5.2
作者：
Ross,R;Raines,E;Bowen-Pope,D
通讯作者：
Bowen-Pope,D
影响因子：
20.3
作者：
D. Phillips;I. Charo;L. Parise;L. Fitzgerald
通讯作者：
D. Phillips;I. Charo;L. Parise;L. Fitzgerald
冯维勒布兰德氏病血管段上血小板粘附减少：初始血小板粘附缺陷。
DOI：
--
发表时间：
1983
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Turitto,VT;Weiss,HJ;Baumgartner,HR
通讯作者：
Baumgartner,HR
血小板粘附到受损冠状动脉：正常猪和冯维勒布兰德病猪的比较。
DOI：
10.1073/pnas.79.16.5076
发表时间：
1982
影响因子：
11.1
作者：
Reddick,RL;Griggs,TR;Lamb,MA;Brinkhous,KM
通讯作者：
Brinkhous,KM
影响因子：
56.9
作者：
PYTELA, R;PIERSCHBACHER, MD;RUOSLAHTI, E
通讯作者：
RUOSLAHTI, E