GROWTH OF THE HEART MUSCLE CELL
心肌细胞的生长
基本信息
- 批准号:3361584
- 负责人:
- 金额:$ 13.87万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1991
- 资助国家:美国
- 起止时间:1991-09-01 至 1994-08-31
- 项目状态:已结题
- 来源:
- 关键词:DNA replication cell differentiation cyclic nucleoside monophosphate cytoskeletal proteins gene expression genetic regulation genetically modified animals laboratory rat mass tissue /cell culture myocardial infarction myogenesis neoplastic cell plasminogen activator protein kinase C protooncogene ventricular hypertrophy
项目摘要
The goal of this research project is to learn more about the regulation of
cardiac muscle cell proliferation, differentiation and hypertrophy using
both neonatal and adult rat primary culture systems and transgenic
transplant tumor atrial myocytes. We are especially interested in the
mechanisms involved with the reinitiation of DNA synthesis in the
terminally differentiated adult cell when placed in culture and as
stimulated by TPA and the reinitiation of DNA synthesis in transgenic mice
myocytes. Another major objective is to learn more about how the structure
of these heart muscle cells relates to their function using our preliminary
results with TPA and cyclic AMP as models. The rationale being to alter
structure and look for changes in function. The specific aims are: 1) To
establish the temporal order of expression of protooncogenes,
muscle-specific, cell cycle-specific, and cytoskeleton genes when neonatal
cardiac muscle cells are placed in culture and as influenced by cyclic AMP.
2) To establish the temporal order of expression of the genes in 1) when
adult cardiac muscle cells are placed in culture during the period of
dedifferentiation and redifferentiation and as influenced by TPA. 3) To
determine the expression of these genes in transgenic myocytes at various
phases during the cell cycle. 4) To characterize temporally the
ultrastructural changes which are occurring in these cell culture systems
and how these changes in ultrastructure correlate with the temporal pattern
of expression of the genes specified in 1). 4) To compare the expression of
these genes in these different culture systems to each other and to the
differentiating in vivo cell to determine if we can establish a pattern of
expression of these genes which will indicate how they may function in the
control of the proliferation and differentiation of the cardiac myocyte.
What we would like to do is establish a cause and effect relationship
between structure and function. If we can understand the mechanisms which
control the differentiation and proliferation of the heart muscle cell,
then it may be possible to design procedures which can be used to initiate
or expedite repair or regeneration of the adult myocardium following injury
such as that caused by a myocardial infraction.
本研究项目的目标是了解更多关于
心肌细胞增殖、分化和肥大
新生和成年大鼠原代培养系统和转基因
移植肿瘤心房肌细胞。 我们特别感兴趣的是
与DNA合成的重新启动有关的机制,
当置于培养物中时,
TPA刺激转基因小鼠DNA合成的重新启动
肌细胞 另一个主要目标是更多地了解结构如何
这些心肌细胞的功能与它们的功能有关,
以TPA和cAMP为模型,理由是改变
结构和功能的变化。 具体目标是:(1)
建立原癌基因表达的时间顺序,
肌肉特异性、细胞周期特异性和细胞骨架基因,
将心肌细胞置于培养物中并受环AMP的影响。
2)为了建立1)中基因表达的时间顺序,当
将成年心肌细胞置于培养物中,
脱分化和再分化以及TPA的影响。3)到
测定这些基因在转基因心肌细胞中的表达,
细胞周期中的各个阶段。4)为了在时间上表征
这些细胞培养系统中发生的超微结构变化
以及这些超微结构的变化如何与时间模式相关联
1)中指定的基因的表达。4)要比较的表达式
这些基因在不同的培养系统中相互作用,
分化体内细胞,以确定我们是否可以建立一种模式,
这些基因的表达,这将表明它们如何在细胞中发挥作用。
控制心肌细胞的增殖和分化。
我们要做的是建立因果关系
结构和功能之间的关系。 如果我们能理解
控制心肌细胞的分化和增殖,
那么就有可能设计出一些程序,
或加速损伤后成人心肌的修复或再生
例如由心肌梗塞引起的。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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WILLIAM C CLAYCOMB其他文献
WILLIAM C CLAYCOMB的其他文献
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{{ truncateString('WILLIAM C CLAYCOMB', 18)}}的其他基金
Potential Use of ES Cells for Cardiac Tissue Engineering
ES 细胞在心脏组织工程中的潜在用途
- 批准号:
7339853 - 财政年份:2007
- 资助金额:
$ 13.87万 - 项目类别:
Potential Use of ES Cells for Cardiac Tissue Engineering
ES 细胞在心脏组织工程中的潜在用途
- 批准号:
7195909 - 财政年份:2007
- 资助金额:
$ 13.87万 - 项目类别:
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