MOLECULAR GENETIC ANALYSIS OF BLOOD PRESSURE IN THE RAT
MOLECULAR GENETIC ANALYSIS OF BLOOD PRESSURE IN THE RAT
批准号:
3364354
负责人:
JOHN P RAPP
金额:
$19.02万
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-07-01 至 1995-06-30
关键词:
DNA alleles angiotensin /renin /aldosterone hypertension angiotensinogen familial hypertension genetic manipulation genetic polymorphism genetic strain genetically modified animals genotype laboratory rat linkage mapping nucleic acid structure peptidyl dipeptidase A receptor renin restriction fragment length polymorphism spontaneous hypertensive rat
中文摘要
我的长期目标是识别和描述导致
不同品系高血压病患者的血压差异
血压正常的大鼠。这项工作是理解其作用机制的基础。
人类遗传性高血压。
这里主要关注的是肾素基因。我已经建立了重要的影响
Dahl盐性高血压患者肾素等位基因的血压研究
敏感(S)大鼠和Dahl耐盐高血压(R)大鼠。肾素
这些菌株携带的等位基因,分别命名为S和r。
在基因实验中与血压共分离。对…的影响
我最近发现的另外两个肾素等位基因的血压
将通过将每个新的等位基因与S的等位基因进行比较来评估。这是
通过确定血压的某一组分是否与
来自Dahl盐敏感的F2群体中的新肾素等位基因
(S)大鼠和一种携带新等位基因的菌株。关于的结构信息
已知的肾素等位基因将被扩展,并搜索其他
在更多品系的老鼠中存在等位基因。
将进行转基因实验以确定S和r肾素
等位基因实际上是导致血压差异的原因。候补方案
可能是这些S和r肾素等位基因与血液共分离
压力,因为它们与一个未知的基因相关联,事实上,该基因导致
血压差。S和r肾素等位基因将插入
利用转基因技术培育盐敏感(S)大鼠品系。这将是
允许评估这些等位基因对血压的影响
独立于连锁的基因座。S和r肾素等位基因的成分可以是
在通过转基因插入的DNA构建物中交换,以便
定位这些等位基因中的DNA多态,这在
造成血压变化。
最后,肾素-血管紧张素系统的其他组成部分(血管紧张素原,
转换酶,由ma癌基因编码的血管紧张素受体)将
评估大鼠品系之间的DNA多态,以及
多态基因将被测试是否与血压存在协同隔离。
英文摘要
My long-term goal is to identify and describe the genes which cause
differences in blood pressure among various strains of hypertensive and
normotensive rats. This work is basic to understanding the mechanism of
genetic hypertension in man.
The main focus here is the renin gene. I have established important effects
on blood pressure for the renin alleles from Dahl salt-hypertension
sensitive (S) rats and Dahl salt-hypertension resistant (R) rats. Renin
alleles carried by these strains, designated s and r respectively,
cosegregated with blood pressure in genetic experiments. The effect on
blood pressure of two additional renin alleles that I have recently found
will be evaluated by comparing each new allele to the s allele. This is
done by determining if a component of blood pressure cosegregates with a
given new renin allele in an F2 population derived from Dahl salt-sensitive
(S) rats and a strain carrying the new allele. Structural information on
the known renin alleles will be extended and a search made for additional
alleles in more strains of rats.
Transgenic experiments will be performed to determine if the s and r renin
alleles are actually causing differences in blood pressure. The alternate
possibility is that these s and r renin alleles cosegregate with blood
pressure because they are linked to an unknown locus that, in fact, causes
the blood pressure differences. s and r renin alleles will be inserted in
the salt-sensitive (S) rat strain using transgenic techniques. This will
allow evaluation of the blood pressure altering effects of these alleles
independent of linked loci. Components of the s and r renin alleles can be
exchanged in DNA constructs that are inserted transgenically in order to
localize 'the' DNA polymorphism within these alleles that is important in
creating blood pressure changes.
Lastly, other components of the renin-angiotensin system (angiotensinogen,
converting enzyme, the angiotensin receptor coded by the mas oncogene) will
be evaluated for DNA polymorphisms among rat strains, and such
polymorphisms will be tested for cosegregation with blood pressure.
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Genetic Analysis of Renal Disease in SHR and Dahl S Rats
-
批准号:6530759
-
项目类别:
-
资助金额:$25.73万
-
财政年份:2001
-
负责人:JOHN P RAPP
-
依托单位:
Genetic Analysis of Renal Disease in SHR and Dahl S Rats
-
批准号:6315130
-
项目类别:
-
资助金额:$28.78万
-
财政年份:2001
-
负责人:JOHN P RAPP
-
依托单位:
POSITIONAL CLONING OF BLOOD PRESSURE QTL
-
批准号:6159456
-
项目类别:
-
资助金额:$21.44万
-
财政年份:2000
-
负责人:JOHN P RAPP
-
依托单位:
POSITIONAL CLONING OF BLOOD PRESSURE QTL
-
批准号:6649757
-
项目类别:
-
资助金额:$36.75万
-
财政年份:2000
-
负责人:JOHN P RAPP
-
依托单位:
POSITIONAL CLONING OF BLOOD PRESSURE QTL
-
批准号:6527523
-
项目类别:
-
资助金额:$36.75万
-
财政年份:2000
-
负责人:JOHN P RAPP
-
依托单位:
FINE GENETIC MAPPING OF BLOOD PRESSURE QTL
-
批准号:6302400
-
项目类别:
-
资助金额:$19.94万
-
财政年份:2000
-
负责人:JOHN P RAPP
-
依托单位:
POSITIONAL CLONING OF BLOOD PRESSURE QTL
-
批准号:6390823
-
项目类别:
-
资助金额:$36.75万
-
财政年份:2000
-
负责人:JOHN P RAPP
-
依托单位:
FINE GENETIC MAPPING OF BLOOD PRESSURE QTL
-
批准号:6110567
-
项目类别:
-
资助金额:$19.94万
-
财政年份:1999
-
负责人:JOHN P RAPP
-
依托单位:
FINE GENETIC MAPPING OF BLOOD PRESSURE QTL
-
批准号:6273124
-
项目类别:
-
资助金额:$18.84万
-
财政年份:1998
-
负责人:JOHN P RAPP
-
依托单位:
FINE GENETIC MAPPING OF BLOOD PRESSURE QTL
-
批准号:6242561
-
项目类别:
-
资助金额:$18.11万
-
财政年份:1997
-
负责人:JOHN P RAPP
-
依托单位:
MOLECULAR GENETIC ANALYSIS OF BLOOD PRESSURE
-
批准号:3364355
-
项目类别:
-
资助金额:$18.52万
-
财政年份:1990
-
负责人:JOHN P RAPP
-
依托单位:
MOLECULAR GENETIC ANALYSIS OF BLOOD PRESSURE IN THE RAT
-
批准号:3364353
-
项目类别:
-
资助金额:$18.73万
-
财政年份:1990
-
负责人:JOHN P RAPP
-
依托单位:
MOLECULAR GENETIC ANALYSIS OF BLOOD PRESSURE
-
批准号:2222101
-
项目类别:
-
资助金额:$20.99万
-
财政年份:1990
-
负责人:JOHN P RAPP
-
依托单位:
MOLECULAR GENETIC ANALYSIS OF BLOOD PRESSURE
-
批准号:3364356
-
项目类别:
-
资助金额:$20.18万
-
财政年份:1990
-
负责人:JOHN P RAPP
-
依托单位:
MOLECULAR GENETIC ANALYSIS OF BLOOD PRESSURE
-
批准号:2222102
-
项目类别:
-
资助金额:$22.92万
-
财政年份:1990
-
负责人:JOHN P RAPP
-
依托单位:
HYPERTENSION
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批准号:3540719
-
项目类别:
-
资助金额:$14.83万
-
财政年份:1988
-
负责人:JOHN P RAPP
-
依托单位:
HYPERTENSION
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批准号:3540717
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项目类别:
-
资助金额:$14.45万
-
财政年份:1988
-
负责人:JOHN P RAPP
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依托单位:
BIOCHEMISTRY AND GENETICS OF HYPERTENSION
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批准号:3485671
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项目类别:
-
资助金额:$37.49万
-
财政年份:1988
-
负责人:JOHN P RAPP
-
依托单位:
BIOCHEMISTRY AND GENETICS OF HYPERTENSION
-
批准号:3336059
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项目类别:
-
资助金额:$34.62万
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财政年份:1988
-
负责人:JOHN P RAPP
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依托单位:
BIOCHEMISTRY AND GENETICS OF HYPERTENSION
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批准号:3336061
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项目类别:
-
资助金额:$37.43万
-
财政年份:1988
-
负责人:JOHN P RAPP
-
依托单位:
海外基金