MECHANISMS OF DAMAGE CAUSED BY CARDIOPULMONARY BYPASS

当白细胞暴露在激活性刺激下时，能够广泛地 在凝血事件中非常重要的各种反应。这个 这些细胞与其他血液成分的相互作用，如 蛋白质、血液因子和血小板会影响血栓的数量。 血管系统受损后形成的组织。 生物材料与血液的相互作用也会引发一系列的凝血。 导致人造表面诱发血栓形成的事件。虽然 已经进行了广泛的研究来确定血小板和 蛋白质在血栓的形成和栓塞术中，已经只有 关于白细胞如何调节这些反应的研究有限。 我们建议研究外周血白细胞在体内的作用。 暴露TE细胞的血液-材料体外相互作用 具有明显不同表面性质的生物材料。这个 白细胞对黏附、吞噬、脱颗粒和 将评估促凝血剂和纤溶活性的表达。 此外，生物材料对补体的激活，以及 随后的白细胞聚集将被调查。 在平行实验中，已被证明是 与生物材料的血栓形成相关的材料将被预吸附到 暴露于白细胞前的聚合物表面，以检查其影响 对白细胞的反应。 花生四烯酸引起的白细胞与血小板的相互作用 将对代谢进行研究，以确定这种代谢物的作用。 在细胞内增强血栓形成的作用。 犬的体外实验将提供有关急性和 白细胞对聚合物材料植入的慢性反应 通过测定白细胞沉降率和 形态学。 这些关于白细胞反应和血栓形成的研究将导致 更好地理解人工智能所涉及的机制 表面诱导血栓形成并将有助于改进设计 生物相容的聚合物材料。

Leukocytes, when exposed to activating stimuli, are capable of a wide variety of responses that are important in coagulation events. The interactions of these cells with other blood components such as proteins, blood factors, and platelets affect the amount of thrombus formation that occurs after damage to the vascular system. The biomaterial-blood interaction also initiates a series of coagulation events that result in artificial surface-induced thrombosis. Although extensive research has been done to determine the role of platelets and proteins in thrombus formation and embolization, there has been only limited study of how leukocytes modulate these responses. We propose to investigate the role of peripheral blood leukocytes in blood-material interactions in vitro by exposing the cells te biomaterials which have markedly different surface properties. The leukocyte responses of adherence, phagocytosis, degranulation, and expression of procoagulant and fibrinolytic activity will be assessed. In addition, the activation of complement by biomaterials, and subsequent leukocyte aggregation will be investigated. In parallel experiments, plasma proteins which have been shown to be relevant to biomaterial thrombogenicity will be preadsorbed onto the polymer surfaces before exposure to leukocytes to examine their effect on the leukocyte response. Leukocyte interactions with platelets as a result of arachidonic acid metabolism will be investigated to determine the role of this metabolite in the cellular enhancement of thrombogenesis. Canine ex vivo experiments will provide information about the acute and chronic reaction of leukocytes to implantation of a polymer material through measurement of leukocyte deposition and morphology. These studies of leukocyte responses and thrombus formation will lead to a better understanding of the mechanisms involved in artificial surface-induced thrombogenesis and will aid in the design of improved biocompatible polymeric materials.