OLIGODENDROGLIA: MYELINATION AND DEMYELINATION

少突胶质细胞可以从新鲜羊脑或牛脑中大量分离 皮质下白色物质或人类尸检组织。 纯化的均相 细胞的制备可以保持为悬浮培养物 完整且可行 这些细胞的代谢非常活跃， 各种脂质和蛋白质，特别是那些富含髓鞘的脂质和蛋白质。 在培养过程中，细胞在细胞周围形成螺旋状的膜 面 对这些膜涡进行纯化和分析后发现， 它们与成熟的致密髓鞘具有共同的性质，尽管 也有明显的差异。 这些膜轮可能是 髓磷脂 为了研究在少突胶质细胞质膜中发现的蛋白质，许多 技术将被使用。 这些包括表面标记探针，凝集素结合， 和免疫沉淀方法。 因此，蛋白质共同的大脑和独特的， 将鉴定少突胶质细胞。 这些调查的结果将 然后将其应用于人类脱髓鞘的情况， 在分子水平上发生在疾病过程中。

Oligodendroglia can be isolated in bulk from fresh lamb or bovine brain subcortical white matter or from human autopsy tissue. The purified homogeneous preparation of cells can be maintained as suspension cultures where they remain intact and viable. The cells are metabolically very active and synthesize a variety of lipids and proteins, especially those found enriched in myelin. While in culture the cells elaborate whorls of membrane adjacent to the cell surface. After purification and analysis of these membrane whorls, it was found they have properties in common with mature compacted myelin, although there are also distinct differences. Possibly these membrane whorls are an early form of myelin. To study the proteins found in the oligodendroglial plasma membranes, many techniques will be used. These include surface labeling probes, lectin binding, and immunoprecipitation methods. Thus protein common to brain and unique to oligodendroglia will be identified. The findings from these investigations will then be applied to human conditions of demyelination to study the events occurring during the disease process at a molecular level.