OLIGODENDROGLIA: MYELINATION AND DEMYELINATION

从新鲜羊脑或牛脑中可大量分离出少突胶质细胞 皮质下白质或来自人类尸检组织。纯净的同质 细胞的制备可以以悬浮培养的形式保持，在那里它们仍然存在 完好无损，还能生存。这些细胞在新陈代谢方面非常活跃，并合成一种 多种脂类和蛋白质，尤指那些富含髓鞘的。 在培养过程中，细胞形成与细胞相邻的螺旋状的膜。 浮出水面。经过对这些膜螺纹的纯化和分析，发现 它们与成熟的致密髓鞘具有共同的特性，尽管有 也有明显的差异。可能这些膜螺纹是一种早期的 髓鞘。 为了研究在少突胶质细胞质膜中发现的蛋白质，许多 将使用各种技术。这些包括表面标记探针、凝集素结合、 和免疫沉淀法。因此，蛋白质对大脑是共同的，对 将会鉴定出少突胶质细胞。这些调查的结果将 然后将其应用于人体脱髓鞘情况的研究 在疾病过程中发生在分子水平上。

Oligodendroglia can be isolated in bulk from fresh lamb or bovine brain subcortical white matter or from human autopsy tissue. The purified homogeneous preparation of cells can be maintained as suspension cultures where they remain intact and viable. The cells are metabolically very active and synthesize a variety of lipids and proteins, especially those found enriched in myelin. While in culture the cells elaborate whorls of membrane adjacent to the cell surface. After purification and analysis of these membrane whorls, it was found they have properties in common with mature compacted myelin, although there are also distinct differences. Possibly these membrane whorls are an early form of myelin. To study the proteins found in the oligodendroglial plasma membranes, many techniques will be used. These include surface labeling probes, lectin binding, and immunoprecipitation methods. Thus protein common to brain and unique to oligodendroglia will be identified. The findings from these investigations will then be applied to human conditions of demyelination to study the events occurring during the disease process at a molecular level.