CLONAL LINES OF THE NERVOUS SYSTEM

这个项目的长期目标是(1)分离祖细胞 中枢神经系统细胞类型的细胞；(2)表征这些细胞 形态和生物化学，包括鉴定 祖细胞特异性抗原(和血清学试剂的发展 对它们的攻击)用于随后的操纵；以及(3)使用这些 研究种群早期发育的规律 将祖细胞群体转化为其直系后代。作为第一步， 少突胶质前体细胞将作为模型系统进行研究， 利用实验室目前的技术能力 髓鞘来源的标志物。经过分离和鉴定后， 少突胶质细胞前体细胞，他们的可能性将被测试 可用于导致实验性脱髓鞘的重新髓鞘形成 文化和动物模型系统，当然，通过引申，类似 这些技术可以应用于人类脱髓鞘疾病的治疗。这个 实验将利用各种细胞分离技术，细胞类型 特定的生化和血清学标记，混合细胞型和富集型 原代细胞培养和克隆细胞系，包括神经胶质细胞、神经元和 畸胎癌细胞群体。

The long term objectives of this project are to (1) isolate progenitor cells of central nervous system cell types; (2) characterize these cells morphologically and biochemically, including the identification of progenitor-cell specific antigens (and development of serological reagents against them) for their subsequent manipulation; and (3) use these populations to study the regulation of the early development of the progenitor cell populations into their immediate progeny. As a first step, the oligodendrocyte progenitor cells will be studied as a model system, taking advantage of the current technical capacity of the laboratory for myelinogenic markers. After isolation and characterization of oligodendrocyte progenitor cells, the possibility will be tested that they can be used to bring about remyelination of experimentally demyelinated culture and animal model systems, and by extention, of course, that similar techniques could be applied to human demyelinating disease therapy. The experiments will utilize a variety of cell separation techniques, cell-type specific biochemical and serological markers, mixed cell type and enriched primary cell culture, and clonal cell lines, including glial, neuronal, and teratocarcinoma cell populations.