CLONAL LINES OF THE NERVOUS SYSTEM

神经系统的克隆系

• 批准号: 3394335
• 负责人: STEVEN E PFEIFFER
• 金额: $ 11.64万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-01-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3394335
• 关键词: astrocytes; carcinoma; cell differentiation; cell growth regulation; cell type; central nervous system; clone cells; electron microscopy; enzyme linked immunosorbent assay; histochemistry /cytochemistry; immunofluorescence technique; mixed tissue /cell culture; myelination; myelinopathy; neoplastic cell; neurochemistry; neurotrophic factors; oligodendroglia; radioimmunoassay; serology /serodiagnosis

The long term objectives of this project are to (1) isolate progenitor cells of central nervous system cell types; (2) characterize these cells morphologically and biochemically, including the identification of progenitor-cell specific antigens (and development of serological reagents against them) for their subsequent manipulation; and (3) use these populations to study the regulation of the early development of the progenitor cell populations into their immediate progeny. As a first step, the oligodendrocyte progenitor cells will be studied as a model system, taking advantage of the current technical capacity of the laboratory for myelinogenic markers. After isolation and characterization of oligodendrocyte progenitor cells, the possibility will be tested that they can be used to bring about remyelination of experimentally demyelinated culture and animal model systems, and by extention, of course, that similar techniques could be applied to human demyelinating disease therapy. The experiments will utilize a variety of cell separation techniques, cell-type specific biochemical and serological markers, mixed cell type and enriched primary cell culture, and clonal cell lines, including glial, neuronal, and teratocarcinoma cell populations.

本项目的长期目标是（1）分离祖细胞 中枢神经系统细胞类型的细胞;（2）表征这些细胞 形态学和生物化学，包括鉴定 祖细胞特异性抗原（和血清学试剂的开发 （3）为他们的后续操作;（3）使用这些 研究早期发育的调节 祖细胞群转化为它们的直系后代。 作为第一步， 少突胶质细胞祖细胞将作为模型系统进行研究， 利用实验室目前的技术能力， 髓鞘生成标记物。 经过分离和鉴定， 少突胶质祖细胞，将测试它们是否可能 可用于使实验性脱髓鞘的 文化和动物模型系统，当然， 该技术可应用于人类脱髓鞘疾病的治疗。 的 实验将利用多种细胞分离技术、细胞类型 特异性生化和血清学标记，混合细胞类型和富集 原代细胞培养和克隆细胞系，包括神经胶质细胞、神经元细胞和 畸胎瘤细胞群。