SALIVA NERVE GROWTH FACTOR, THE NATURALLY OCCURRING FORM

唾液神经生长因子，天然存在的形式

• 批准号: 3396823
• 负责人: DAVID M YOUNG
• 金额: $ 4.61万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-09-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396823
• 关键词: X ray crystallography; analytical ultracentrifugation; atomic absorption spectrometry; biological products; cell migration; chemical structure; chemotaxis; cinematography; complement pathway; electrofocusing; fluorescence spectrometry; gel electrophoresis; gel filtration chromatography; human tissue; ion exchange chromatography; neurotrophic factors; neutrophil; radiotracer; saliva; tissue /cell culture; wound healing; zinc

Several lines of evidence now indicate that nerve growth factor isolated from mouse salivary glands displays multiple biologic functions which span several health-related functions. These activities include: 1) activation of plasminogen; 2) promotion of the rate of wound healing; 3) neurite outgrowth stimulatory activity; 4) activation of the classical pathway of complement; and 5) chemotactic activity toward human leukocytes. The research proposal contained herein is designed to enhance our understanding of each of these potential biologic roles of NGF. Experiments are designed to further our understanding of the chemical mechanism whereby NGF undergoes autocatalytic activation - and its control by zinc ion. Studies are also presented which aim to understand the isolation of NGF from Cohn Fraction III of human serum and from bovine seminal plasma. Finally, a major goal of the laboratory is to explore and to understand our recent finding that nerve growth factor is strongly chemotactic for polymorphonuclear leukocytes and for monocytes. These studies, together with our finding that NGF can participate directly in activation of the complement system, may contribute significantly to our understanding of the role of NGF in the wound healing process.

现在有几条证据表明神经生长因子分离 来自小鼠唾液腺显示多种生物学功能 几个与健康相关的功能。 这些活动包括：1）激活 纤溶酶原; 2）促进伤口愈合率； 3）神经突 出生刺激活性； 4）激活经典途径 补充; 5）对人白细胞的趋化活性。 本文包含的研究建议旨在增强我们的 理解NGF的每个潜在生物学作用。 实验旨在进一步了解化学物质 NGF经历自催化激活及其控制的机制 由锌离子。 还提出了旨在了解NGF隔离的研究 来自人类血清的Cohn III和牛的精确血浆。 最后，实验室的主要目标是探索和了解我们的 最近的发现，神经生长因子对 多形核白细胞和单核细胞。 这些研究一起 我们发现NGF可以直接参与激活 补体系统，可能会对我们对 NGF在伤口愈合过程中的作用。