BIOENERGETIC REGULATION AND NEUROLOGICAL MATURATION

本申请中描述的研究旨在表征 丙酮酸脱氢酶多酶复合物的调节特性 老鼠的大脑 通过丙酮酸脱氢酶反应的代谢通量， 酶复合物的活化状态和磷酸化水平 将在线粒体和突触体中测定酶的活性。 从大脑的几个单独区域提取的制剂 从成年大鼠和大鼠在其早期发育阶段。 的 丙酮酸脱氢酶或丙酮酸代谢的调节通常 在来自成年大鼠和发育中的灌注脑中进行研究 大鼠 激素和/或营养物质改变的影响 动物状态对丙酮酸调节参数的影响 脱氢酶复合物将在该代谢制剂中进行研究。 这个研究项目的长期目标是记录关键的 大脑调节其重要功能的能力 能量产生途径和神经功能的维持 在这个组织里。

The research described in this application is designed to characterize the regulatory properties of the pyruvate dehydrogenase multienzyme complex in the rat brain. Metabolic flux through the pyruvate dehydrogenase reaction, the activation state of the enzyme complex and the level of phosphorylation of the enzyme will be determined in mitochondrial and synaptosomal preparations derived from several individual regions of the brain taken from adult rats and from rats during their early developmental stages. The regulation of pyruvate dehydrogenase or pyruvate metabolism in general will be investigated in perfused brains derived from adult rats and developing rats. The effects of alterations in the hormal and/or the nutritional state of the animal on the regulatory parameters for the pyruvate dehydrogenase complex will be investigated in this metabolic preparation. The long range goal of this research project is to document the crucial relationship between the ability ofthe brain to regulate its important energy generating pathways and the maintenance of neuro-logical competence in this tissue.