NEURONS CONTAINING ACETYLCHOLINE & NEUROACTIVE PEPTIDES

神经细胞个体产生和释放单一的 所有终端的神经递质化学物质都是一种简化 几十年来许多神经科学的概念。最近的发现 一些神经细胞显然会产生一种以上的物质 作用于其他神经细胞和肌肉的能力，以及进一步的一些 这些物质是多肽，与已建立的 神经递质引发了一些关于基本的 神经系统中的沟通和整合机制。我们有 在大脑中发现了两对容易识别的神经元B11和B12 腹足软体动物Tritonia的中枢神经系统 生产和释放至少两种神经活性物质。其中一名特工是 乙酰胆碱和另一种至少是一种肽。相对简单的 三叉神经症的神经系统含有异常大的神经元，可以 细胞内刺激和记录，并允许分析特定的 神经细胞之间的电和化学相互作用。它也是 有可能确定单个神经元在 完整的动物的行为。在这份提案中，描述了实验 为了确认我们最初发现的B11包含和使用的内容，可以单独 或者是乙酰胆碱和多肽类神经递质的组合。也是, 我们将尝试确定这些神经元在行为和行为方面的功能 蜂窝术语。

The idea that nerve cells individually produce and release a single neurotransmitter chemical from all their terminals has been a simplifying concept for much of neuroscience for several decades. The recent findings that some nerve cells apparently produce more than one substance with the capacity to act on other nerve cells and muscles, and further that some of these substances are peptides quite unlike the established neurotransmitters, have provoked a number of questions about the basic mechanisms of communication and integration in the nervous system. We have discovered two pairs of readily identifiable neurons, B11 and B12 in the central nervous system of the gastropod mollusk, Tritonia which apparently produce and release at least two neuroactive agents. One of these agents i acetylcholine and the other is at least one peptide. The relatively simple nervous system of Tritonia contains unusually large neurons which can be stimulated and recorded intracellularly, and permits analysis of specific electrical and chemical interactions between nerve cells. It is also possible to determine the role played by the individual neurons in the behavior of the intact animal. In this proposal, experiments are described to confirm our initial findings that B11 contain and use, either separately or in combination, acetylcholine and peptide neurotransmitters. As well, we will try to determine the function of these neurons in behavioral and cellular terms.