CONFOCAL FLUORESCENCE SCANNING IMAGING SYSTEM

共焦荧光扫描成像系统

• 批准号: 3521980
• 负责人: A DENNIS WILLOWS
• 金额: $ 14.4万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-30 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521980
• 关键词: biomedical equipment purchase; biomedical equipment resource; confocal scanning microscopy; fluorescence; fluorescence microscopy; fluorescent dye /probe

This is a proposal to establish a laser confocal fluorescence microscope at the Friday Harbor Laboratories of the University of Washington. It will be used by several NIH supported resident research programs and, as well, by other visiting investigators from U.S. and overseas institutions. We propose combining a BioRad MRC-600 confocal system and a Zeiss Axiovert microscope for qualitative and quantitative imaging of structurally complex sections, whole mount and living material. Specific examples of work proposed are: 1) (A. Gordon) Localization of fluorescently labelled troponin C and myosin regulatory light chain in skinned muscle fibers will be used to determine molecular mechanisms of regulation of muscle contraction. 2) (J. Blinks) A study of inositol triphosphate-induced release of CA++ in skinned muscle fibers. Is IP3, acting as an intracellular chemical messenger, the "missing link" in excitation contraction coupling? In a separate project, the photocytes of luminescent cnidaria will be studied to determine the subcellular localization of the green fluorescent protein. 3) (A.O.D. Willows) Developmental tracking of fluorescence-labeled neurons and axonal processes having serotonin and peptide antigenicity will be used to answer questions about the assembly of neural subsystems during ontogeny of the central nervous system. The instrument will also support the work of many other investigators whose research is directly dependent upon the biological resources and instrumentation of the Friday Harbor Laboratories. FHL, located 75 air miles NW of Seattle on San Juan Island, is the marine biological laboratory of the University of Washington. It serves as a national and international resource for diverse marine scientists interested in topics including cell and molecular biology, physiology, and biomedicine.

这是建立激光共焦荧光显微镜的提案 在华盛顿大学星期五港实验室。 它 将由 NIH 支持的多个驻地研究项目使用，并且 来自美国和海外机构的其他来访调查人员。 我们建议结合使用 BioRad MRC-600 共焦系统和 Zeiss Axiovert 用于结构定性和定量成像的显微镜 复杂截面、整体安装和生活材料。 具体例子 拟议的工作是：1）（A. Gordon）荧光标记的定位 皮肌纤维中的肌钙蛋白 C 和肌球蛋白调节轻链将 用于确定肌肉调节的分子机制 收缩。 2) (J. Blinks) 三磷酸肌醇诱导的研究 皮肤肌纤维中 CA++ 的释放。 是IP3，充当 细胞内化学信使，激发中的“缺失环节” 收缩耦合？在一个单独的项目中，发光的光细胞 将研究刺胞动物以确定其亚细胞定位 绿色荧光蛋白。 3) (A.O.D. Willows) 发育追踪 荧光标记的神经元和轴突具有血清素和 肽抗原性将用于回答有关组装的问题 中枢神经系统个体发育期间的神经子系统。这 仪器还将支持许多其他研究人员的工作，他们 研究直接依赖于生物资源和 星期五港实验室的仪器。 FHL 位于西雅图西北 75 英里处的圣胡安岛，是海军陆战队 华盛顿大学生物实验室。 它充当 为不同海洋科学家提供的国家和国际资源 对细胞和分子生物学、生理学等主题感兴趣 生物医学。