DESCENDING CONTROL OF SOMATOSENSORY INPUT

拟议的研究将涉及三个方面的下降控制 体感输入，它涉及几个高级神经解剖学 技巧。在一个项目中，我们将对组织进行研究 猫皮质下行传入楔状核的轴突内分布 辣根过氧化物酶染色。光学显微镜和电子显微镜 不同来源个体皮质纤维及其终末的研究 将进行功能区调查，以获取与 大脑皮质通过以下方式调节信息处理的机制 楔形神经元。拟议研究的另一部分旨在 对延髓腹底复合体中惰性神经元的假设的验证 猫是GABA能的，通过免疫细胞化学和 放射自显影技术，脑内突触关系 丘脑躯体感觉中继器。第三个项目将采用精选的 氨基酸逆行转运研究皮质神经元 对猫的腹基复合体可能使用谷氨酸或天冬氨酸作为 神经递质和验证选择性逆行的假设 运输是一种可靠的实验方法，用于识别 神经递质由给定途径使用的神经递质。

The proposed research will deal with three aspects of descending control of somatosensory input and it involves several advanced neuroanatomical techniques. In one project we will pursue a research on the organization of descending cortical input to the cuneate nucleus of cats by intraaxonal staining with horseradish peroxidase. Light- and electro microscopical study of individual cortical fibers and their terminals from different functional areas will be carried out to obtain evidence relevant to the mechanisms by which the cerebral cortex modulates information processing by cuneate neurons. Another part of the proposed research is aimed at verifying the hypothesis that inerneurons in the ventrobasal complex of cats are GABA-ergic and to explore, by immunocytochemical and autoradiographic techniques, synaptic relationships within the somatosensory thalamic relay. A third project will employ the selective retrograde transport of amino acids to investigate whether cortical neurons to the ventrobasal complex of cats may use glutamate or aspartate as neurotransmitters and to verify the assumption that selective retrograde transport is a reliable experimental approach for the identification of the neurotransmitter used by a given pathway.