COMPLEX GLYCOLIPIDS OF BRAIN AND RELATED SYSTEMS

提供有关鞘糖脂在体内代谢的信息，使用 培养的皮肤成纤维细胞来自正常供者和神经病供者 脂肪储存障碍、高雪氏病和泰萨克氏病 从这些细胞中分离、纯化和鉴定缺陷的 水解酶、调节蛋白和激活辅助因子，使用 层析和免疫学技术。这些信息应该会导致 旨在合理控制这些破坏性疾病的步骤。 研究β-半乳糖苷特异性凝集素及其复合体的作用 鞘糖脂在鸡胚成肌细胞融合培养中的作用机制 以及竞争性低聚糖、凝集素和致病物质的作用 蛋白质，利用糖鞘糖脂的特定标记技术 凝集素的活体亲和层析和免疫学研究 身份识别，以便达成一种纠正 肌肉营养不良和多发性硬化症的缺陷。 探讨突触唾液酸酶-唾液酸蛋白-唾液酸脂复合体的作用 在神经递质的结合和释放中，通过特定的标记，通过 亲和层析、干扰及成分表征 哺乳动物大脑中的5-羟色胺能神经末梢。这些发现应该有助于 对大脑功能缺陷及其破坏性影响的理解 迷幻剂和神经毒素。 探讨疱疹病毒转化细胞胞外唾液酸酶在人疱疹病毒感染中的作用 培养，使用层析和特定的放射性示踪技术 示踪该酶对细胞表面唾液酸代谢的影响 以及后者对参与细胞外分泌的胞外酶的控制作用。 神经细胞鸟苷环化酶激活剂乙酰胆碱的分解。这 信息应该有助于接近合理的病毒治疗方法 诱发的神经病变和神经致瘤性。

To provide information on the metabolism of glycosphingolipids in vivo, using cultured skin fibroblasts from normal donors and donors with the neurological lipid-storage disorders, Gaucher's and Tay-Sachs Diseases with emphasis upon isolation from these cells, purification, and characterization of the deficient hydrolases, regulatory proteins, and activating co-factors, using chromatographic and immunologic techniques. This information should lead to steps aimed at a logical control of these destructive diseases. To investigate the role of beta-galactoside-specific lectin and complex glycosphingolipids in the mechanism of chick embryo myoblast fusion in culture, and the effects of competitive oligosaccharides, lectins, and pathogenic proteins, utilizing techniques for specific labelling of glycosphingolipids in vivo and affinity chromatographic and immunological approach to lectin identification, so as to arrive at an approach toward the correction of the defects in muscular dystrophies and multiple sclerosis. To investigate the role of synaptic sialidase-sialoprotein-sialolipid complexes in neurotransmitter binding and release by specifically labelling, isolating by affinity chromatography, disruption and componential characterization of serotonergic nerve endings from mammalian brain. The findings should aid in an understanding of faulty brain function and the destructive effects of hallucinogens and neurotoxins. To investigate the role of ecto-sialidase of herpes-virus transformed cells in culture, using chromatographic and specific radioactive tracer techniques to trace the effects of this enzyme on the metabolism of cell surface sialic acid and the controlling influence of the latter on ecto-enzymes involved in the breakdown of the neural cell guanyl cyclase activator, acetylcholine. This information should be helpful in approaching a rational therapy for virally induced neuropathies and neuro-oncogenicity.