SALIVA NERVE GROWTH FACTOR, THE NATURALLY OCCURRING FORM

现在有几条证据表明，神经生长因子 从小鼠的唾液腺显示出多种生物学功能 几个与健康相关的功能。这些活动包括：1)激活 纤溶酶原；2)促进创面愈合率；3)轴突 生长刺激活性；4)激活经典的刺激生长途径。 补体；5)对人白细胞的趋化活性。 本文中包含的研究建议旨在增强我们的 了解NGF的每一种潜在的生物学作用。 实验的目的是加深我们对这种化学物质的理解 NGF自催化活化的机制及其调控 通过锌离子。 此外，还介绍了旨在了解NGF分离的研究 从人血清的科恩组分III和牛精浆中提取。 最后，实验室的一个主要目标是探索和了解我们的 最近发现神经生长因子对癌症具有强烈的趋化作用 多形核白细胞和单核细胞。这些研究加在一起 随着我们的发现，NGF可以直接参与 补语系统，可能对我们理解补语有很大的帮助 神经生长因子在伤口愈合过程中的作用。

Several lines of evidence now indicate that nerve growth factor isolated from mouse salivary glands displays multiple biologic functions which span several health-related functions. These activities include: 1) activation of plasminogen; 2) promotion of the rate of wound healing; 3) neurite outgrowth stimulatory activity; 4) activation of the classical pathway of complement; and 5) chemotactic activity toward human leukocytes. The research proposal contained herein is designed to enhance our understanding of each of these potential biologic roles of NGF. Experiments are designed to further our understanding of the chemical mechanism whereby NGF undergoes autocatalytic activation - and its control by zinc ion. Studies are also presented which aim to understand the isolation of NGF from Cohn Fraction III of human serum and from bovine seminal plasma. Finally, a major goal of the laboratory is to explore and to understand our recent finding that nerve growth factor is strongly chemotactic for polymorphonuclear leukocytes and for monocytes. These studies, together with our finding that NGF can participate directly in activation of the complement system, may contribute significantly to our understanding of the role of NGF in the wound healing process.