INTERACTIONS BETWEEN H+.1 AND CA2+1 IN MYXICOLA AXON
INTERACTIONS BETWEEN H+.1 AND CA2+1 IN MYXICOLA AXON
批准号:
3399172
负责人:
RONALD F ABERCROMBIE
金额:
$14.2万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1982
资助国家:
美国
项目状态:
已结题
起止时间:
1982-05-01 至 1993-08-31
关键词:
acidity /alkalinity adenosine triphosphate axon axoplasm caffeine calcium binding protein calcium metabolism calcium transporting ATPase cell cycle cell differentiation cell membrane hormone regulation /control mechanism inositol inositol phosphates ion transport mitochondria muscle contraction neurofilament neurofilament proteins organelles physical chemical interaction secretion
中文摘要
长期目标是获得更好的理解
英文摘要
Long-term objectives are to gain a better understanding of the
regulation of both intracellular free calcium (Ca2+) i, and free
protons, (H+), and the interdependence between these two ion
concentrations. These ions are now known or suspected to
regulate or influence numberous cell functions, including muscle
contraction, neuro-secretion, ion permeability, intercellular
electrical coupling, cell division and differentiation, and
metabolism. Calcuium regulatory mechanisms will be examined at
four loci: (a) endoplasmic reticulum, (b) plasma membrane, (c)
intracellular Ca-binding proteins, and (d) mitochondria. Calcium
uptake and release from the endoplasmic reticulum of neural
cytoplasm iwll be characterized using a new Ca electrode that
detects free Ca in the nM concentration range. ATP-dependent
Ca uptake will be studied in the presence of mitochondrial
inhibitors as a function of ATP, pH, and (cA2+). The effects of
caffeine and inositol phosphates on Ca release from organelles
close to the plasma membrane will be compared to organelles
near the center of the axon. Intracellular Ca-binding proteins will
be studied with special emphasis on neurofilaments. The 150,000-
160,000 molecular-weight neurofilament proteins will be isolated
and the binding of Ca to these proteins will be quantitiatively
examined in solutions of different pH, ionic strength, and
temperature. Using limited, specific proteolytic cleavage and
autoradiographic analysis, the portions of the neurofilament
molecule containg the calcium-binding site will be examined. The
influence of pH on mitochondria will be studied. Using
tetraphenol phosphonium (TPP+), a TPP+-microelectrode, and
samples of axoplasm containing mitochondria, the effect of pH on
the mitochondrial membrane potential will be examined to see if
this explains a previus observation concerning pH and Ca uptake.
Using pH microelectrodes and isolated samples of cytoplasm, the
"effective" hydrogen ion diffusion coefficient in neural cytoplasm
will be determined, and the importance of fixed and mobile pH
buffers in dissipating intracellular pH gradients will be evaluated.
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INTRACELLULAR H+ AND CA++ IN MYXICOLA AXON
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批准号:2263544
-
项目类别:
-
资助金额:$17.47万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTRACELLULAR H+ AND CA++ IN MYXICOLA AXON
-
批准号:3399173
-
项目类别:
-
资助金额:$17.46万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTERACTIONS BETWEEN H-1 AND CA2-1 IN MYXICOLA AXON
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批准号:3399179
-
项目类别:
-
资助金额:$15.61万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTERACTIONS BETWEEN H-1 AND CA2-1 IN MYXICOLA AXON
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批准号:3399176
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项目类别:
-
资助金额:$13.3万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTERACTIONS BETWEEN H+ AND CA+ IN AXON
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批准号:3399175
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项目类别:
-
资助金额:$8.91万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTERACTIONS BETWEEN H-1 AND CA2-1 IN MYXICOLA AXON
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批准号:3399177
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项目类别:
-
资助金额:$13.56万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTERACTIONS BETWEEN H+ AND CA+ IN AXON
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批准号:3399174
-
项目类别:
-
资助金额:$7.83万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTERACTIONS BETWEEN H-1 AND CA2-1 IN MYXICOLA AXON
-
批准号:3399178
-
项目类别:
-
资助金额:$14.55万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTERACTIONS BETWEEN H+ AND CA+ IN AXON
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批准号:3399171
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项目类别:
-
资助金额:$8.64万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTRACELLULAR H+ AND CA++ IN MYXICOLA AXON
-
批准号:2263545
-
项目类别:
-
资助金额:$18.22万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
INTRACELLULAR H+ AND CA++ IN MYXICOLA AXON
-
批准号:2263543
-
项目类别:
-
资助金额:$17.25万
-
财政年份:1982
-
负责人:RONALD F ABERCROMBIE
-
依托单位:
海外基金