PHYSIOLOGY OF MUSCARINIC SYNAPSES IN SYMPATHETIC GANGLIA

交感神经节传递信号， 对压力的反应。 该项目的长期目标是 了解正常交感神经系统的两个基本方面 physiology. 第一个目标是确定 交感神经元，选择性地支配不同种类的神经 外围目标（例如，血管与腺体）和第二个 目的是确定慢突触的整合作用， 交感神经节中的传导。 潜在的假设 交感神经元的功能亚类释放 共发射器的不同组合来控制它们的 各自的目标和减缓神经节中的突触电位 用于调节重复动作电位的激发 从而提供了一种用于调节 来自给定类别的神经节细胞的单独共递质。 这个项目最重要的方面是提供一个模型 了解毒蕈碱的生理意义， 自主神经节中的肽能突触。 另外这款 研究将开发一种体外系统， 和调节表型特性的分子机制 功能上不同的交感神经元。 使用牛蛙，结合电生理和 解剖学方法将用于解决四个具体目标： 1)验证交感神经C细胞是血管性的假设 利用肾上腺素（EPI）、神经肽Y（NPY）和 三磷酸腺苷（ATP）作为共递质。 2)确定毒蕈碱和肽能突触电位 以及它们产生的重复放电， 调节C神经元释放EPI、NPY和ATP， 动脉 3)开发一种组织培养系统， 可以识别，并使用膜片钳方法， 表征产生和调节的膜电流 动作电位的重复放电 4)检验快B和/或慢B但不是C型的假设 交感神经元支配皮肤粘液腺， 颗粒腺 然后找到合适的解剖标记， 可用于追踪B型神经元的外周投射， 鉴定组织培养中分离的B神经元

Sympathetic ganglia convey signals that mediate adaptive responses to stress. The long-term goals of this project are to understand two fundamental aspects of normal sympathetic physiology. The first goal is to identify functional subsystems of sympathetic neurons that innervate selectively different classes of peripheral targets (eg. blood vessels vs. glands) and the second goal is to determine the integrative role of slow synaptic transmission in sympathetic ganglia. The underlying hypotheses are that functional subclasses of sympathetic neurons release different combinations of co-transmitters to control their respective targets and that slow synaptic potentials in ganglia serve to modulate the firing of repetitive action potentials thereby providing a mechanism for regulating the release of individual co-transmitters from a given class of ganglion cells. The most important aspect of this project is to provide a model for understanding the physiological significance of muscarinic and peptidergic synapses in autonomic ganglia. In addition, this research will develop an in vitro system for identifying cellular and molecular mechanisms that regulate phenotypic properties of functionally distinct sympathetic neurons. Using bullfrogs, a combination of electrophysiological and anatomical methods will be used to address four specific aims: 1) Test the hypothesis that sympathetic C cells are vasomotor neurons that utilize epinephrine (EPI), neuropeptide Y (NPY) and adenosine triphosphate (ATP) as co-transmitters. 2) Determine how muscarinic and peptidergic synaptic potentials in ganglia and the repetitive firing that they engender, each regulate the release of EPI, NPY and ATP from C neurons onto arteries. 3) Develop a tissue culture system in which a vasomotor C cells can be identified and the use patch clamp methods to characterize the membrane currents that produce and modulate the repetitive firing of action potentials. 4) Test the hypothesis that fast B and/or slow B but not C-type sympathetic neurons innervate cutaneous mucous glands and granular glands. Then find suitable anatomical markers that can be used to trace the peripheral projections of B-type neurons and to identify dissociated B neurons in tissue culture