PHYSIOLOGY OF MUSCARINIC SYNAPSES IN SYMPATHETIC GANGLIA

交感神经节传递信号，介导适应性 对压力的反应。这个项目的长期目标是 理解正常交感的两个基本方面 生理学。第一个目标是确定以下功能的子系统 选择性地支配不同类别的交感神经元 外围目标的数量(例如血管与腺体)和第二个 目的是确定慢突触的整合作用 在交感神经节中传递。潜在的假设 交感神经元的功能亚类是否释放 不同的同轴发送器组合以控制其 神经节的各个靶点和慢突触电位 用于调节重复动作电位的激发 从而提供了一种调节释放的机制 来自特定类别神经节细胞的单个共递质。 这个项目最重要的方面是提供一个模型 了解毒扁豆碱的生理学意义 自主神经节中的肽能突触。此外，这一点 研究人员将开发一种用于识别细胞的体外系统 以及调节表型特性的分子机制 功能截然不同的交感神经元。 使用牛蛙，电生理和 解剖学方法将被用来解决四个具体目标： 1)检验交感C细胞是血管舒缩因子的假设 利用肾上腺素(EPI)、神经肽Y(NPY)和 三磷酸腺苷(ATP)作为共递质。 2)确定毒鼠碱和多肽能突触电位 在神经节和它们产生的重复激发中，每一个 调节C区神经元EPI、NPY和ATP的释放 动脉。 3)开发一种组织培养系统，在该系统中血管运动C细胞 可以识别并使用膜片钳方法来 描述产生和调制的膜电流 动作电位的重复激发。 4)测试快B和/或慢B但不是C型的假设 交感神经元支配皮肤粘液腺和 颗粒腺。然后找到合适的解剖学标记， 用来追踪B型神经元的外周投射和 组织培养中分离的B细胞的鉴定

Sympathetic ganglia convey signals that mediate adaptive responses to stress. The long-term goals of this project are to understand two fundamental aspects of normal sympathetic physiology. The first goal is to identify functional subsystems of sympathetic neurons that innervate selectively different classes of peripheral targets (eg. blood vessels vs. glands) and the second goal is to determine the integrative role of slow synaptic transmission in sympathetic ganglia. The underlying hypotheses are that functional subclasses of sympathetic neurons release different combinations of co-transmitters to control their respective targets and that slow synaptic potentials in ganglia serve to modulate the firing of repetitive action potentials thereby providing a mechanism for regulating the release of individual co-transmitters from a given class of ganglion cells. The most important aspect of this project is to provide a model for understanding the physiological significance of muscarinic and peptidergic synapses in autonomic ganglia. In addition, this research will develop an in vitro system for identifying cellular and molecular mechanisms that regulate phenotypic properties of functionally distinct sympathetic neurons. Using bullfrogs, a combination of electrophysiological and anatomical methods will be used to address four specific aims: 1) Test the hypothesis that sympathetic C cells are vasomotor neurons that utilize epinephrine (EPI), neuropeptide Y (NPY) and adenosine triphosphate (ATP) as co-transmitters. 2) Determine how muscarinic and peptidergic synaptic potentials in ganglia and the repetitive firing that they engender, each regulate the release of EPI, NPY and ATP from C neurons onto arteries. 3) Develop a tissue culture system in which a vasomotor C cells can be identified and the use patch clamp methods to characterize the membrane currents that produce and modulate the repetitive firing of action potentials. 4) Test the hypothesis that fast B and/or slow B but not C-type sympathetic neurons innervate cutaneous mucous glands and granular glands. Then find suitable anatomical markers that can be used to trace the peripheral projections of B-type neurons and to identify dissociated B neurons in tissue culture