PHYSIOLOGY OF MUSCARINIC SYNAPSES IN SYMPATHETIC GANGLIA

交感神经节传递命令，实现对身体的反应 压力。该项目的长期目标是了解三个 慢的突触电位有助于正常的整合功能 牛蛙交感神经节，并找出调节 毒鼠型突触在神经节中的表达。的工作假设 这一建议是：1)神经节含有生理特化的和 相互独立的功能通道，调制不同类别的 外周终末器官，2)慢电位使神经节细胞产生 活动的特征模式，以最佳地推动其特定的 靶点和3)神经节细胞的突触连接调节它们的 毒鼠型突触的表达。含有以下成分的分离制剂 第九和第十椎旁神经节将被用来研究毒鼠强 兴奋性突触后电位(EPSP) 突触电位(IPSP)与亮氨酸化介导的肽能性EPSP 激素释放激素(LHRH)。三种生理上可识别的类型 这些神经节内神经元(快B、慢B、C)与内脏的联系 下腹部(如膀胱)和后肢的其他靶点(例如 外分泌腺、血管系统、感觉感受器)。然而，这种关系 在这些细胞类型和假设的功能通道之间 不确定。 将使用电生理和解剖学方法相结合的方法 为了提供对神经节突触的详细描述， 已识别的交感神经元和已识别的细胞向 皮肤、运动和内脏分支或周围神经。接下来，报道 毒扁豆碱类EPSP的电压敏感度是不同的 寻求确定毒碱兴奋的异质性是如何相关的 与B细胞亚类的关系以及它们与M细胞的不同之处 对重复射击的影响。然后毒扁豆碱抑制重复性 触发将通过电压钳位C单元进行分析。在全系列之后 已经表征了毒鼠碱对重复激发的调制， 神经节、脊髓和外周的特殊联系对其的影响 神经节细胞毒碱反应差异表达的研究 在轴突切断、去神经和再神经支配的过程中，将研究其类型。

Sympathetic ganglia convey commands that implement reactions to bodily stress. The long-term goals of this project are to understand how three slow synaptic potentials contribute to the normal integrative function of bullfrog sympathetic ganglia and to identify factors that regulate the expression of muscarinic synapses in ganglia. The working hypotheses for this proposal are that 1) ganglia contain physiologically specialized and antomically separate functional channels that modulate different classes of peripheral end organs, 2) slow potentials enable ganglion cells to generate characteristic patterns of activity to drive optimally their specific targets and 3) the synaptic connections of ganglion cells regulate their expression of muscarinic synapses. Isolated preparations containing the ninth and tenth paravertebral ganglia will be used to study the muscarinic excitatory postsynaptic potential (epsp), the muscarinic inhibitory post synaptic potential (ipsp) and a peptidergic epsp mediated by leuteinizing hormone releasing hormone (LHRH). Three physiologically identifiable types of neurons (fast B, slow B, C) within these ganglia innvervate viscera in the lower abdomen (e.g. bladder) and other targets in the hindlimbs (e.g. exocrine glands, vasculature, sensory receptor). However, the relation between these cell types and the hypothesized functional channels is uncertain. A combination of electrophysiological and anatomical methods will be used to provide a detailed description of ganglionic synapses, the morphology of identified sympathetic neurons and the projections of identified cells into cutaneous, motor and visceral branches or peripheral nerves. Next, reports that muscarinic epsps are variable in their voltage-sensitivity will be pursued to determine how heterogeneity in muscarinic excitation is related to the subclasses of B cells and how muscarinic epsps differ in their effects upon repetitive firing. Then muscarinic inhibition of repetitive firing will be analyzed by voltage clamping C cells. After the full range of muscarinic modulation of repetitive firing has been characterized, the influence of specific connections between the ganglia, cord and periphery upon the differential expression of muscarinic responses in ganglion cell types will be studied during axotomy, denervation and re-innervation.