CA2+,H+, AND NEUROSECRETION IN BRAIN NERVE TERMINALS

CA2、H 和脑神经末梢的神经分泌

• 批准号: 3400797
• 负责人: DANIEL A NACHSHEN
• 金额: $ 7.23万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-12-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3400797
• 关键词: acid base balance; brain cell; calcium metabolism; cations; fluorescence spectrometry; fluorescent dye /probe; homeostasis; membrane permeability; membrane potentials; nerve endings; neurophysiology; neurotransmitters; radiotracer; secretion; spectrometry; synapses; synaptosomes

The goal of the proposed research is to understand some of the fundamental properties of mammalian presynaptic nerve terminals. Pinched-off nerve endings (synaptosomes) isolated from rat brain will be used, because they retain many functional, metabolic, and morphological properties of intact neuronal tissue, and are well-suited for studies that involve radiotracer-flux, optical, and biochemical techniques. The Investigator will: 1) examine the characteristics of the presynaptic Ca channels involved in neurosecretion - permeability and selectivity, and mechanism of inactivation; 2) determine how cations that permeate, and selectively block, Ca channels influence Ca sequestration and extrusion and transmitter release; 3) measure intracellular pH in the nerve terminals, study how it is regulated, and how it is related to Ca channel activity and neurosecretion; 4) develop and utilize methods for loading Ca-indicators into nerve terminals, so that Ca metabolism and its relation to the activity of nerve terminals can be investigated. This project will provide a better understanding of the basic cellular mechanisms that control intracellular Ca and pH in brain nerve endings, and of how this regulation affects the relase of brain neurotransmitters.

拟议研究的目标是了解一些基本的