NEURONAL ORGANIZATION OF THE BASOLATERAL AMYGDALA

这项拟议的研究是长期调查的第一部分， 神经元，神经化学和突触组织的外侧和 基底外侧核（L-BL）的大鼠杏仁核。 的主要目标 该建议是1）表征细胞类型， 生长抑素，CCK，VIP，脑啡肽和GABA; 2）描述了罚款 包括终端类型的不同小区类别的结构 3）确定每种细胞类型的相对数量 在L-BL的每个细分; 4）检查地形组织 投射到不同大脑区域的神经元; 5）决定细胞 （6）估计当地居民的百分比; 电路神经元在每个细分;和7）检查肽 投射到不同脑区的神经元的细胞化学。 的 用于实现这些目的的技术包括高尔基技术， 免疫细胞化学，逆行轴突运输法，联合 免疫细胞化学-HRP轴突运输技术，电子 显微镜 细胞类型的分析将涉及细胞类型的相关性。 不同类型的高尔基体浸渍神经元的形态学 用其他技术染色。 将使用各种统计技术 以促进不同技术之间的相关性。 光 拟议研究的微观部分将阐明 L-BL的结构、形态学和细胞化学组织。 的 电子显微镜研究启动了长期的超微结构 分析这些核。 本实验室未来的研究将结合联合收割机 高尔基体，免疫细胞化学，逆行轴突运输和轴突 变性技术与电子显微镜，以进一步澄清 神经元，细胞化学和突触组织的外侧和 基底外侧核 当这些信息与 电生理学和药理学数据，可能最终表明新的 缓解过度攻击性行为和时间性行为等疾病的方法 癫痫的治疗方法有哪些？ 杏仁核

The proposed study is the initial part of a long-term investigation of the neuronal, neurochemical, and synaptic organization of the lateral and basolateral nucleus (L-BL) of the rat amygdala. The major objectives of this proposal are to 1) characterize the cell types that contain somatostatin, CCK, VIP, enkephalin, and GABA; 2) describe the fine structure of different cell classes including the types of terminals that contact their perikarya; 3) determine the relative number of each cell type in each subdivision of L-BL; 4) examine the topographical organization of neurons that project to different brain areas; 5) determine the cell type(s) involved in these projections; 6) estimate the percentage of local circuit neurons in each subdivision; and 7) examine the peptide cytochemistry of neurons projecting to different brain areas. The techniques used to accomplish these aims include the Golgi technique, immunocytochemistry, retrograde axonal transport methods, combined immunocytochemical-HRP axonal transport technique, and electron microscopy. Analysis of cell types will involve a correlation of the morphology of different classes of Golgi-impregnated neurons with neurons stained by other techniques. Various statistical techniques will be used to facilitate correlations between different techniques. The light microscopic portion of the proposed study will elucidate basic aspects of the structural, hodological, and cytochemical organization of L-BL. The electron microscopic investigation initiates a long-term ultrastructural analysis of these nuclei. Future studies in this laboratory will combine Golgi, immunocytochemical, retrograde axoal transport and axonal degeneration techniques with electron microscopy to further clarify the neuronal, cytochemical, and synaptic organization of the lateral and basolateral nuclei. This information, when correlated with electrophysiological and pharmacological data, may ultimately suggest novel ways to alleviate disorders such as hyperaggressive behavior and temporal lobe epilepsy by pharmacologically manipulating the activity of the amygdala.