SYNAPTIC ORGANIZATION OF THE BASOLATERAL AMYGDALA

基底外侧杏仁核的突触组织

• 批准号: 6188294
• 负责人: ALEXANDER JOSEPH MCDONALD
• 金额: $ 21.27万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188294
• 关键词: GABA receptor; amines; amygdala; calcium binding protein; cholecystokinin; dopamine receptor; electron microscopy; electrophysiology; glutamate receptor; immunocytochemistry; interneurons; light microscopy; neural information processing; nicotinamide adenine dinucleotide; norepinephrine; serotonin receptor; somatostatin; synapses; vasoactive intestinal peptide

DESCRIPTION (Adapted From The Applicant's Abstract): The proposed study is the beginning of a long-term investigation of the synaptic organization of the basolateral amygdala that will focus on the basal amygdalar nucleus (BAN). The amygdala is one of the chief forebrain regions concerned with emotion. Clinical interest in the basolateral amygdala stems from its involvement in temporal lobe epilepsy, Alzheimer's disease, and psychiatric disorders (including anxiety, depression, and schizophrenia). It is also one of the main regions targeted by benzodiazepines and other psychiatric medications. Despite the importance of this brain region, very little is known about the synaptic organization of its constituent psychiatric disorders involving the amygdala. The proposed studies will focus on the inhibitory interneurons of the BAN. These investigations will use immunocytochemical methods at the light and electron microscopic levels to identify the main subpopulations of inhibitory interneurons and to analyze their connections: 1) with the principal neurons of the BAN, 2) with the serotonergic, dopaminergic, and noradrenergic afferents to the BAN, and 3) with each other. The synaptic connections of electrophysiological characterized principal neurons and interneurons will also be examined; this represents the first time that electrophysiological and ultrastructural techniques will be combined to elucidate the synaptic organization of the basolateral amygdala. In addition, it will be determined if particular interneuronal subpopulations in the BAN exhibit specific subtypes of GABAergic, glutamatergic, and monoaminergic receptors, and whether there are extensive non-synaptic interactions of monoaminergic transmitters with BAN neurons.III.

描述（根据申请人的摘要改编）： 拟议的研究是对长期研究的开始 基底外侧杏仁核的突触组织，将重点放在基础上 杏仁核核（禁令）。杏仁核是主要的前脑区域之一 与情感有关。基底外侧杏仁核茎的临床兴趣 从参与颞叶癫痫，阿尔茨海默氏病和 精神疾病（包括焦虑，抑郁和精神分裂症）。这是 这也是苯二氮卓类和其他精神病的主要区域之一 药物。尽管这个大脑地区很重要，但知之甚少 关于其成分精神疾病的突触组织 涉及杏仁核。拟议的研究将集中于抑制 禁令的中间神经元。这些调查将使用免疫细胞化学 光和电子显微镜水平的方法以识别主要 抑制性中间神经元的亚群并分析其连接：1） 与该禁令的主要神经元一起，2）具有血清素能，多巴胺能， 和非肾上腺素能传入，以及3）彼此之间。突触 电生理特征的主要神经元的连接和 中间神经元也将进行检查；这代表了第一次 电生理和超微结构技术将合并到 阐明基底外侧杏仁核的突触组织。此外， 将确定该禁令中的特定神经元亚群是否 表现出特定的GABA能，谷氨酸能和单胺能的亚型 受体，以及是否存在广泛的非突触相互作用 带有BAN NEURONS.III的单胺能发射器。