CALCIUM-ACTIVATED NEUTRAL PROTEINASE IN CNS

这个项目探索了一种假设，即钙激活的中性 蛋白水解酶(S)(CANP)与髓鞘和/或定位于轴突 和神经胶质细胞。本论文是从我们的演示中发展而来的 钙介导的细胞骨架和髓鞘蛋白的降解 由脊髓和脑白质中存在的一种蛋白酶(S)所致。因此，我们的 将进行详细的研究1)以确定CANP与 髓鞘：我们计划检测不同区域的酶(CANP)活性 在发育过程中，大脑的髓鞘和亚细胞部分 和来自不同物种的中枢神经系统和三叉神经节的髓鞘，以及2)检查 抗血清免疫细胞化学技术定位CANP的研究 来自纯化的CANP(我们最近从牛身上提纯 大脑)。这种酶在髓鞘和相关结构中的存在或 细胞可能在脱髓鞘过程中髓鞘的分解中起关键作用 疾病(如多发性硬化症)。

This project explores the hypothesis that a calcium-activated neutral proteinase(s) (CANP) is associated with myelin and/or localized in axons and glial cells. This thesis has been developed from our demonstration of a calcium-mediated degradation of cytoskeletal as well as myelin proteins by a proteinase(s) present in spinal cord and brain white matter. Thus our detailed studies will be 1) to establish that CANP is associated with myelin: we plan to examine the enzyme (CANP) activity in different regions of brain, in myelin and subcellular fractions of brain during development and myelin from CNS and PNS of different species, and 2) to examine the localization of CANP by immunocytochemical technique after antisera is raised from purified CANP (which we have recently purified from bovine brain). The presence of this enzyme in myelin and related structures or cells may play a crucial role in the breakdown of myelin in demyelinating diseases (e.g. MS).