SYNAPTIC ORGANIZATION OF BASAL FOREBRAIN PROJECTIONS

这项研究的目的是详细建立生理 成人基底前脑向边缘系统的输入特性 大鼠 三个具体的途径将被研究：（1）预测从 Broca斜角带核到后扣带回皮质， (2)从斜角带到内嗅皮质的投射，以及（3） 腹侧苍白球/基底核向基底外侧的投射 杏仁核 这些途径将由 活体细胞内记录的神经生理学技术， 标签。 基底前脑会被电刺激激活， 并且神经元反应将在靶细胞内被记录 地区 扣带皮层和内嗅皮层中的靶神经元以及 杏仁核将通过标记它们来进行形态学鉴定， 细胞内注射辣根过氧化物酶（HRP）。 基底 丘脑-扣带回突触传递的前脑调制 将研究预测。 最后一个目标是将基底前脑 神经元细胞内与HRP，以详细描述其 轴突投射和共侧化的模式。 这些研究将建立在体内生理和形态 基底节广泛投射系统与基底节广泛投射系统的关系 前脑和边缘系统的三个重要组成部分， 扣带皮层（海马的重要皮层靶点之一） 形成），内嗅皮层（输入的主要来源， 齿状回和海马）和杏仁核。 这些细胞内 因此，这些研究将填补我们对突触的认识中的重大空白。 这些系统的组织和生理学。

The objective of this research is to establish in detail the physiological properties of inputs from the basal forebrain to the limbic system in adult rats. Three specific pathways will be studies: (1) projections from the nucleus of the diagonal band of Broca to the posterior cingulate cortex, (2) projections from the diagonal band to the entorhinal cortex, and (3) projections from the ventral pallidum/nucleus basalis to the basolateral nucleus of the amgydala. The pathways will be studied by the neurophysiological techniques of in vivo intracellular recording and labeling. The basal forebrain will be activated by electrical stimulation, and neuronal responses will be recorded intracellularly in the target areas. Target neurons in the cingulate and entorhinal cortices and the amygdala will be morphologically identified by labeling them with intracellular injections of horseradish peroxidase (HRP). The basal forebrain modulation of synaptic transmission in thalamo-cingulate projections will be studied. A final goal is to inject basal forebrain neurons intracellularly with HRP in order to characterize in detail their pattern of axonal projections and coilateralization. The studies will establish the in vivo physiological and morphological relationship between the extensive projection system of the basal forebrain, and three important components of the limbic system, the cingulate cortex (one of the important cortical targets of the hippocampal formation), the entorhinal cortex (a principal source of input to the dentate gyrus and hippocampus), and the amygdala. These intracellular studies will thus fill significant gaps in our knowledge about the synaptic organization and physiology of these systems.