CALCIUM-ACTIVATED NEUTRAL PROTEINASE IN CNS

中枢神经系统中钙激活的中性蛋白酶

• 批准号: 3402433
• 负责人: NAREN L BANIK
• 金额: $ 7.92万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-02-01 至 1989-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3402433
• 关键词: axon; calcium; cell type; central nervous system; electrophoresis; glia; histochemistry /cytochemistry; immunochemistry; isozymes; myelin; organelles; peptidases; peripheral nervous system; radioassay

This project explores the hypothesis that a calcium-activated neutral proteinase(s) (CANP) is associated with myelin and/or localized in axons and glial cells. This thesis has been developed from our demonstration of a calcium-mediated degradation of cytoskeletal as well as myelin proteins by a proteinase(s) present in spinal cord and brain white matter. Thus our detailed studies will be 1) to establish that CANP is associated with myelin: we plan to examine the enzyme (CANP) activity in different regions of brain, in myelin and subcellular fractions of brain during development and myelin from CNS and PNS of different species, and 2) to examine the localization of CANP by immunocytochemical technique after antisera is raised from purified CANP (which we have recently purified from bovine brain). The presence of this enzyme in myelin and related structures or cells may play a crucial role in the breakdown of myelin in demyelinating diseases (e.g. MS).

该项目探讨了钙激活中性物质的假设 蛋白酶 (CANP) 与髓磷脂相关和/或位于轴突中 和神经胶质细胞。 本论文是根据我们的论证发展而来的 钙介导的细胞骨架和髓磷脂蛋白降解 由存在于脊髓和脑白质中的蛋白酶产生。 因此我们的 详细研究将是 1) 确定 CANP 与 髓磷脂：我们计划检查不同区域的酶（CANP）活性 大脑的髓鞘质和发育过程中大脑的亚细胞部分 以及来自不同物种的 CNS 和 PNS 的髓磷脂，以及 2) 检查 抗血清后通过免疫细胞化学技术定位 CANP 从纯化的 CANP 中提取（我们最近从牛中纯化） 脑）。 这种酶存在于髓磷脂和相关结构中或 细胞可能在脱髓鞘过程中髓磷脂的分解中发挥关键作用 疾病（例如多发性硬化症）。